An international team involving researchers from the University and University Hospital Zürich, the Krembil Research Institute and the University and University Hospital in Toronto (Canada), the Department of Physics of Jyväskylä (Finland), the University of Leuven (Belgium), the Johannes Kepler University in Linz (Austria), the Novartis Institutes for Biomedical Research in Emeryville (USA), the ETH Zürich and the Paul Scherrer Institute has developed a protocol that enables hierarchical imaging and computational analysis of vascular networks in entire postnatal- and adult mouse brains, enabling direct and quantitative comparisons of the morphological brain vascular network architecture between different postnatal and / or adult developmental stages. The results have been published on Nature Protocols on September 3rd, 2021.