PXII - X10SA: Macromolecular Crystallography
Beamline for protein crystallography
The second beamline for protein crystallography (PX) has been jointly funded by the Max Planck Society (MPG) and the pharmaceutical companies Novartis and Hoffmann La Roche in 2005. It is a highly versatile PX beamline with good compromise of small X-ray focus and low divergence, making it suitable for the high-throughput data collection of our pharmaceutical partners as well as the high-impact academic research performed by the MPG. After more than ten years of operation and more than 1500 structures deposited to the PDB, with some ten thousand proprietary structures solved, we recently took on four more partners: Boehringer Ingelheim, Proteros, LeadXpro and SAI. These partners and the Paul Scherrer Institut are therefore the principal users of this facility. The design and construction of beam line X10SA (PXII) is based on the highly successful first beam line X06SA (PXI) and is particularly suited for large unit cells and small crystals. In 2017 we introduce a novel approach for fast micro-focusing using X-ray lenses, giving users the ability to use a true micro-beam on-demand.
As an option that can be applied for by all users, crystallographic data collection can be complemented by optical spectroscopy with an on-axis microspectrophotometer (c.f. R. Pompidor et al., JSR, (2013), 20, p765).
The second beamline for protein crystallography (PX) has been jointly funded by the Max Planck Society (MPG) and the pharmaceutical companies Novartis and Hoffmann La Roche in 2005. It is a highly versatile PX beamline with good compromise of small X-ray focus and low divergence, making it suitable for the high-throughput data collection of our pharmaceutical partners as well as the high-impact academic research performed by the MPG. After more than ten years of operation and more than 1500 structures deposited to the PDB, with some ten thousand proprietary structures solved, we recently took on four more partners: Boehringer Ingelheim, Proteros, LeadXpro and SAI. These partners and the Paul Scherrer Institut are therefore the principal users of this facility. The design and construction of beam line X10SA (PXII) is based on the highly successful first beam line X06SA (PXI) and is particularly suited for large unit cells and small crystals. In 2017 we introduce a novel approach for fast micro-focusing using X-ray lenses, giving users the ability to use a true micro-beam on-demand.
As an option that can be applied for by all users, crystallographic data collection can be complemented by optical spectroscopy with an on-axis microspectrophotometer (c.f. R. Pompidor et al., JSR, (2013), 20, p765).
Beamline Characteristics
| Wavelength range (Å) | 0.62 - 2.07 |
|---|---|
| Spectral range (keV) | 6.0 - 20.0 |
| Energy resolution (%) | <0.02 |
| Flux at 12.4 keV at 400mA (ph/s) | >2 x 1012 |
| Focused spot size h x v (μm²) | 73x16 / 30x16 / 10x10 |
| Detector | PILATUS 6M (25 Hz) |
Beamline Features
Fast On-Demand Beam Shaping
- Using apertures (150μm; 30μm; 10μm)
- Beam size selection while sample is already mounted (<5 sec)
Beamsizes X10SA Feb 2016
On-axis in-situ Micro-Spectrophotometry
- Permanently installed on-axis micro-spectrophotometer
- big portfolio of excitation Lasers
- Possible techniques:
- UV/Vis
- Raman
- Resonant Raman
- Fluorescence
Beamspots of the Micro-Photospectrometer X10SA
Beamline Partners
Beamline Partners X10SA as of January 2019