Full field tomography detection system
- Optical microscope
- Visible light camera (detector)
Various combinations of different scintillators, microscopes, and cameras are available at TOMCAT to achieve the optimal image quality for a given experimental setup. The choice of equipment depends mainly on the desired magnification, spatial resolution, and field of view, as well as the X-ray energy to be used and the required acquisition speed. The following paragraphs give some technical details and specifications of the available components.
Other scintillator options are available. If you need a specialized setup, please contact beamline staff in advance of your experiments. Users are also welcome to bring their own scintillators, but should contact beamline staff in advance to ensure that the scintillator fits onto the microscope and a proper mount is available and ready to use.
Microscope 1: Standard high resolution microscope (Optique Peter)This microscope system is based on diffraction-limited optics and can accommodate 1.25x, 2x, 4x, 10x, 20x and 40x objectives as listed in the table below. The field-of-view and effective pixel sizes are calculated in the standard configuration, i.e., for the pco.EDGE 5.5 detector with a pixel size of 6.5 μm. If a different detector is used, these numbers will change accordingly based on the detector's pixel size.
|Objective||Magnification||Numerical Aperture||Field-of-view (mm2)||Pixel Size (μm2)|
|PLAPO1.25x||1.25||0.06||13.3 x 11.2||5.2 x 5.2|
|PLAPO2x||2||0.08||8.3 x 7.0||3.25 x 3.25|
|UPLAPO4x||4||0.16||4.2 x 3.5||1.63 x 1.63|
|UPLAPO10x||10||0.40||1.7 x 1.4||0.65 x 0.65|
|UPLAPO20x||20||0.70||0.8 x 0.7||0.33 x 0.33|
|UPLAPO40x||40||0.90||0.4 x 0.3||0.16 x 0.16|
Microscope 2: 1:1 Optics (Optique Peter)This system is based on a high numerical aperture tandem 1:1 configuration, accepting a diagonal up to 40 mm. It is typically used for the DPC setup or for large samples that do not require high spatial resolution. A larger pixel size option is available within this setup. Please contact the beamline staff if this option would benefit your experiments.
|Lens||Magnification||Focal length||Field-of-view (mm2)||Pixel Size (μm2)|
|KinoOptik||1.0||150 mm||16.6 x 14.0||6.5 x 6.5|
Microscope 3: 2-4x Continuous Magnification White-Beam Microscope (Elya Solutions)This microscope features a continuously adjustable magnification from 2.24 to 3.78 times, and it is designed with a high numerical aperture and for polychromatic radiation. It is typically used for high-speed experiments with both the pco.EDGE 5.5 and the GigaFRoST detectors, either with monochromatic or polychromatic radiation. The table below lists the approximate range of pixel sizes and fields-of-view attainable within the limits of the magnification for the two different detector types.
|Camera||Magnification||Field-of-view (mm2)||Pixel Size (μm2)|
|pco.EDGE 5.5||2.24||7.4 x 6.3||2.9 x 2.9|
|pco.EDGE 5.5||3.78||4.4 x 3.7||1.7 x 1.7|
|GigaFRoST||2.24||9.9 x 9.9||4.9 x 4.9|
|GigaFRoST||3.78||5.9 x 5.9||2.9 x 2.9|
Microscope 4: High Resolution White-Beam Microscope (Optique Peter)
This is a long working distance, high resolution microscope typically used with polychromatic radiation. It is based on diffraction-limited optics and allows the selection of two different magnifications by exchanging the objective lens (5x or 10x). In combination with the 2x eye piece, the effective nominal magnification is 10x or 20x. Removal of the eye piece improves the light throughput significantly, but changes the focal lengths as well. Without the eye piece, the 5x and 10x objective lenses result in effective magnifications of 6.8x and 13.7x, respectively (no longer infinity-corrected).
The two objective lenses are manufactured by Mitutoyo and have the following specs:
|Objective||Magnification||Numerical Aperture||Focal length [mm]|
|M Plan Apo 5x||5.0||0.14||200|
|M Plan Apo 10x||10.0||0.28||200|
|Camera||Magnification||Field-of-view (mm2)||Pixel Size (μm2)|
|pco.EDGE 5.5||6.8x||2.4 x 2.1||0.96 x 0.96|
|pco.EDGE 5.5||13.7x||1.2 x 1.0||0.47 x 0.47|
|GigaFRoST||6.8x||3.25 x 3.25||1.61 x 1.61|
|GigaFRoST||13.7x||1.6 x 1.6||0.80 x 0.80|
Macroscope 5: High Numerical Aperture White-Beam Macroscope (Optique Peter)
This novel, high quality custom-made macroscope offers a 4x magnification and has a very high numerical aperture of 0.35, making it highly efficient. The macroscope is modular, flexible and can be mounted both in a horizontal and vertical configuration, to best match the sample environment and imaging requirements. It has been designed for high-resolution time-resolved X-ray tomographic microscopy and can be operated using both polychromatic and monochromatic radiation, coupled either to the pco.Edge or GigaFRoST detector. The macroscope has a tunable aperture which can be used to optimize the spatial resolution for different imaging setups. The high efficiency and superior image quality of the macroscope have made tomographic studies with a time resolution of 20 Hz and beyond at TOMCAT a reality. A detailed description of the macroscope performance can be found here (DOI: 10.1107/S1600577519004119).
The available fields-of-view and pixel sizes are described in the table below:
|Camera||Field-of-view (mm2)||Pixel Size (μm2)|
|pco.EDGE 5.5||4.2 x 3.5||1.6|
|GigaFRoST||5.5 x 5.5||2.75|
|pco.Edge 5.5||pco.Edge 4.2||pco.Dimax||GigaFRoST|
|Pixel size [μm]||6.5||6.5||11.0||11.0|
|Sensor size [pixels] (h x v)||2560 x 2160||2048 x 2048||2016 x 2016||2016 x 2016|
|Sensor size [Megapixels]||5.5||4.2||4.1||4.1|
|Max frame rate (full frame)|| 100 fps (FS,RS)
33 fps (SS,RS)
| 100 fps (FS)
35 fps (SS)
|1255 fps||1255 fps|
|Max frame buffer (full frame)||3'000||3'000||6’307||71’860|
|Exposure time||500μs - 2s||100μs - 10s||2μs - 40ms||2μs - 40ms|
|Dynamic range [dB]||88.6||90.4||65.8||65.8|
|Dark current [e-]||1.2||1.0||<20||<20|
|Cooling||water (chiller)||water (chiller)||air (fan)||air (fan)|
- RS: Rolling Shutter
- GS: Global Shutter
- FS: Fast scan
- SS: Slow scan
pco.Edge 5.5This is the low noise and large field of view camera by pco, and the work-horse camera for standard measurements at TOMCAT. It is built on sCMOS technology and features a sensor size of 2560 x 2160 pixels, 6.5μm pixel size and a 16-bit nominal dynamic range → technical specifications.
pco.Edge 4.2This is the slightly smaller brother of the pco.Edge 5.5, featuring even slightly lower noise levels, but at the expense of a reduced sensor size (2048 x 2048 pixels). It is also based on sCMOS technology with a 6.5μm pixel size and a 16-bit nominal dynamic range → technical specifications.
pco.DimaxThe pco.Dimax is the high-speed camera offered by pco. The imaging chip is built on CMOS technology and features 2016 x 2016 pixels, 11μm pixel size and a 12-bit nominal dynamic range. The camera has an on-board memory of 36 GB and is read out via a USB2.0 connection (slow!) → technical specifications.
This camera is rarely used at present and mostly replaced by the GigaFRoST camera.
GigaFRoSTThe GigaFRoST camere is a PSI in-house development incorporating the same imaging chip as the pco.Dimax, but featuring a novel readout system providing continuous and sustained data streaming at up to ~8GB/s to a dedicated high-performance data backend server. This allows for the high-speed acquisition of long time series to observe dynamic phenomena in a time-resolved manner during long perdiods of time.
For an in-depth description of the GigaFRoST camera system refer to R. Mokso, C. M. Schlepütz, G. Theidel, H. Billich, E. Schmid, T. Celcer, et al., "GigaFRoST: The Gigabit Fast Readout System for Tomography", J. Synchrotron Rad., 24 (6), 1250-1259 (2017). DOI: 10.1107/S1600577517013522.
Post-Processing and Reconstructions
F. Marone, and M. Stampanoni, "Regridding reconstruction algorithm for real time tomographic imaging", J. Synchrotron Rad., 19, 1029-1037 (2012). pdf