SwissFEL Alvra experimental station
Alvra specializes in measuring the ultrafast dynamics of photochemical and photobiological systems using a variety of X-ray scattering and spectroscopic techniques. Alvra consists of two instruments: Alvra Prime and Alvra Flex. Prime is a helium/low vacuum atmosphere chamber designed to perform a range of techniques, including serial femtosecond crystallography (SFX), X-ray scattering (XDS), and X-ray absorption and emission spectroscopy (XAS and XES) over the full SwissFEL hard X-ray photon energy range of 2-12.4 keV. Flex is a flexible instrument designed to accommodate user experiments with an X-ray spectrometer that can be used for many different types of measurements, including inelastic X-ray scattering (IXS) and high energy resolution off-resonant spectroscopy (HEROS), as well as host user-provided setups. An overview of the Alvra design parameters can be found here and on the SwissFEL facility here. A user laboratory, to support chemistry and biology experiments is also available on-site. The summary of the lab infrastructure and equipment can be found here.
Alvra is currently in full user operation mode, with both the Prime and Flex instruments commissioned at 100 Hz. A summary of the experimental station parameters follows
| Photon energy range | 2 – 12.4 keV (full SwissFEL Aramis photon energy range) |
|---|---|
| Beam profile | Measured focus 20 µm (FWHM) at 2 and 4.5 keV (defocussed beam also possible) |
| Measured focus 2 µm (FWHM) at 12 keV (defocussed beam also possible) | |
| Unfocused beam: 500 µm to 1 mm (FWHM) energy dependent | |
| Flexible KB mirrors can also provide an asymmetric focus | |
| Bandwidth | SASE 0.25% (pink beam) |
| Monochromatic: Si(111), Si(311) and InSb(111) | |
| Sample Environment | From vacuum (5 x 10-4 mbar) up to 800 mbar pressure (He or N2) |
| Sample delivery systems | Liquid jet for chemistry: |
| - Flat jet (100, 200 and 300 µm) with gear or peristaltic pump | |
| - Round jet (20-100 µm) with HPLC pump | |
| LCP viscous injector (50-100 µm) | |
| GDVN injectors tested in chamber | |
| Solid samples | |
| Detectors and Spectrometers | 16M Jungfrau forward scattering detector at 10 cm distance (<1 Å resolution at 12.4 keV) |
| 4M Jungfrau forward scattering detector at 10 cm distance (1.5 Å resolution at 12.4 keV) | |
| 2 x 2 crystal von Hamos dispersive X-ray emission spectrometer (1-12.4 keV) | |
| Diodes for x-ray absorption measurements |
| Photon energy range | 6 – 12.4 keV |
|---|---|
| Beam profile | Flexible focus from 10 to >100 µm (FWHM) |
| Unfocused beam: ~1 x 1 mm2 (FWHM) energy dependent | |
| Bandwidth | SASE 0.5% (pink beam) |
| Monochromatic: Si(111), Si(311) and InSb(111) | |
| Sample Environment | Atmospheric conditions (in-air setup) |
| Sample delivery systems | Liquid jet for chemistry: |
| - Flat jet (100, 200 and 300 µm) with gear or peristaltic pump | |
| - Round jet (20-100 µm) with HPLC pump | |
| LCP viscous injector (50-100 µm) | |
| Solid samples | |
| Detectors and Spectrometers | 3 crystal von Hamos dispersive X-ray emission spectrometer with 1M Jungfrau detector |
| Diodes for x-ray absorption measurements |
| Primary pump source | 800 nm, 35 fs FWHM, 10 mJ (Ti:Sapphire) |
|---|---|
| Secondary pump sources | 240 nm – 2.5 micron (OPA with additional nonlinear conversion options). At present pulse energies at the sample location for 240-400 nm have been measured in the range of 10-30 µJ. We expect pulse energies of 150-500 µJ between 400 nm and 780 nm; 150-500 µJ between 1 µm and 2.5 µm. Pulse durations are expected to be approximately 50 fs fwhm. |
| Timing diagnostics | Alvra currently has two spectral encoding time-tools available, one pre and one post-interaction region. This approach has been demonstrated at several other facilities and works well for experiments that use the full SASE pulses at the full X-ray range of the endstation (2-12 keV) and for monochromatic experiments at the higher x-ray energies (>6 keV). |
Current Highlights and News
How to get chloride ions into the cell
A molecular movie shot at PSI reveals the mechanism of a light-driven chloride pump
A protein's unexpected "doming"
Researchers have coaxed a secret out of the vital protein cytochrome c that it kept well-hidden up to now. Measurements at the X-ray free-electron laser SwissFEL reveal structural changes that science had previously ruled out for this kind of biomolecule.
Advances in de novo protein structure determination using long-wavelength native-SAD phasing at SwissFEL
An international team of scientists from the Paul Scherrer Institute and members of the LeadXpro and Heptares pharmaceutical companies led by Karol Nass (Alvra group, SwissFEL) demonstrated a significant advancement in de novo protein structure determination at X-ray free-electron lasers. Their article, published recently in IUCrJ (DOI: 10.1107/S2052252520011379), describes structure determination of a membrane protein and an important drug target (A2A adenosine receptor) by native single-wavelength anomalous diffraction (native-SAD) at SwissFEL with up to ten fold reduction in the required number of indexed images.