Dr. Chia-Ying Huang

photo of Chia-Ying Huang


Paul Scherrer Institute
Forschungsstrasse 111
5232 Villigen PSI

Research Interests

  • In Meso In Situ Serial Crystallography (IMISX)
  • Membrane Protein Crystallography and Structural Biology
  • Fixed-Target Sample Delivery
  • Method Development for In Situ Data Collection at Cryogenic and Room Temperature
  • Feb. 2019 – present. Scientist in the Macromolecular Crystallography Group. Paul Scherrer Institute/Swiss Light Source, Switzerland.
  • May 2016 – Jan. 2019. Postdoctoral Research Fellow (Paul Scherrer Institute FELLOW-II-3i) in the Macromolecular Crystallography Group. Paul Scherrer Institute/Swiss Light Source, Switzerland.
  • Oct. 2013 – Apr. 2016. Postdoctoral Research Fellow in Prof. Martin Caffrey’s Lab. Trinity Biomedical Sciences Institute, Trinity College Dublin, Ireland.
  • Jul. 2012 – Sep. 2013. Postdoctoral Research Fellow in Dr. Tsai-Ling Yang Lauderdale’ s Lab. National Institute of Infectious Diseases and Vaccinology, National Health Research Institute, Taiwan.
ORCID ID https://orcid.org/0000-0002-7676-0239

-In Meso In Situ Serial X-Ray Crystallography (IMISX)

Huang C-Y, Meier N, Caffrey M, Wang M, Olieric V. 3D-printed holders for in meso in situ fixed-target serial X-ray crystallography. Journal of Applied Crystallography. 2020; 53(3): https://doi.org/10.1107/S1600576720002897

Huang C-Y, Olieric V, Caffrey M, Wang M. In meso in situ serial X-Ray crystallography (IMISX): a protocol for membrane protein structure determination at the Swiss Light Source In: Perez C, Maier T, eds. Expression, purification, and structural biology of membrane proteins. Methods in molecular biology. New York: Humana; 2020. https://doi.org/10.1007/978-1-0716-0373-4_20

Cheng R, Huang C-Y, Hennig M, Nar H, Schnapp G In situ crystallography as an emerging method for structure solution of membrane proteins: the case of CCR2A FEBS Journal. 2020; 287(5): 866-873. https://doi.org/10.1111/febs.15098

Apel A-K, Cheng RKY, Tautermann CS, Brauchle M, Huang C-Y, Pautsch A, et al. Crystal structure of CC chemokine receptor 2A in complex with an orthosteric antagonist provides insights for the design of selective antagonists Structure. 2019; 27(3): 427-438.e5. https://doi.org/10.1016/j.str.2018.10.027

El Ghachi M, Howe N, Huang C-Y, Olieric V, Warshamanage R, Touzé T, et al. Crystal structure of undecaprenyl-pyrophosphate phosphatase and its role in peptidoglycan biosynthesis Nature Communications. 2018; 9(1): 1078 (13 pp.). https://doi.org/10.1038/s41467-018-03477-5

Huang C-Y, Olieric V, Howe N, Warshamanage R, Weinert T, Panepucci E, et al. In situ serial crystallography for rapid de novo membrane protein structure determination. Communications Biology. 2018; 1: 124 (8 pp.). https://doi.org/10.1038/S42003-018-0123-6

Huang C-Y, Olieric V, Ma P, Howe N, Vogeley L, Liu X, et al. In meso in situ serial X-ray crystallography of soluble and membrane proteins at cryogenic temperatures Acta Crystallographica Section D: Structural Biology. 2016; 72(1): 93-112. https://doi.org/10.1107/S2059798315021683

Huang C-Y, Olieric V, Ma P, Panepucci E, Diederichs K, Wang M, et al. In meso in situ serial X-ray crystallography of soluble and membrane proteins Acta Crystallographica Section D: Structural Biology. 2015; 71(6): 1238-1256. https://doi.org/10.1107/S1399004715005210

-Membrane Protein Structural Biology

Olatunji S, Yu X, Bailey J, Huang C-Y, Zapotoczna M, Bowen K, et al. Structures of lipoprotein signal peptidase II from Staphylococcus aureus complexed with antibiotics globomycin and myxovirescin Nature Communications. 2020; 11(1): 140 (11 pp.). https://doi.org/10.1038/s41467-019-13724-y

Crack JC, Amara P, Volbeda A, Mouesca J-M, Rohac R, Pellicer Martinez MT, et al. Electron and proton transfers modulate DNA binding by the transcription regulator RsrR Journal of the American Chemical Society. 2020; 142(11): 5104-5116. https://doi.org/10.1021/jacs.9b12250

Li H, Huang C-Y, Govorunova EG, Schafer CT, Sineshchekov OA, Wang M, et al. Crystal structure of a natural light-gated anion channelrhodopsin eLife. 2019; 8: e41741 (21 pp.). https://doi.org/10.7554/eLife.41741

Yin W, Zhou XE, Yang D, de Waal PW, Wang M, Dai A, et al. Crystal structure of the human 5-HT1B serotonin receptor bound to an inverse agonist Cell Discovery. 2018; 4: 12 (13 pp.). https://doi.org/10.1038/s41421-018-0009-2

Wiktor M, Weichert D, Howe N, Huang C-Y, Olieric V, Boland C, et al. Structural insights into the mechanism of the membrane integral N-acyltransferase step in bacterial lipoprotein synthesis Nature Communications. 2017; 8: 15952 (13 pp.). https://doi.org/10.1038/ncomms15952

-Long-Wavelength Structural Phasing

Basu S, Olieric V, Leonarski F, Matsugaki N, Kawano Y, Takashi T, et al. Long-wavelength native-SAD phasing: opportunities and challenges IUCrJ. 2019; 6(3): 1-14. https://doi.org/10.1107/S2052252519002756

-Beamline Software and Automation

Basu S, Kaminski JW, Panepucci E, Huang C-Y, Warshamanage R, Wang M, et al. Automated data collection and real-time data analysis suite for serial synchrotron crystallography Journal of Synchrotron Radiation. 2019; 26(1): 244-252. https://doi.org/10.1107/S1600577518016570

Wojdyla JA, Panepucci E, Martiel I, Ebner S, Huang C-Y, Caffrey M, et al. Fast two-dimensional grid and transmission X-ray microscopy scanning methods for visualizing and characterizing protein crystals Journal of Applied Crystallography. 2016; 49: 944-952. https://doi.org/10.1107/S1600576716006233