• In Meso In Situ Serial Crystallography (IMISX)
• Membrane Protein Crystallography and Structural Biology
• Fixed-Target Sample Delivery
• Method Development for In Situ Data Collection at Cryogenic and Room Temperature

• Feb. 2019 – present. Scientist in the Macromolecular Crystallography Group. Paul Scherrer Institute/Swiss Light Source, Switzerland.
• May 2016 – Jan. 2019. Postdoctoral Research Fellow (Paul Scherrer Institute FELLOW-II-3i) in the Macromolecular Crystallography Group. Paul Scherrer Institute/Swiss Light Source, Switzerland.
• Oct. 2013 – Apr. 2016. Postdoctoral Research Fellow in Prof. Martin Caffrey’s Lab. Trinity Biomedical Sciences Institute, Trinity College Dublin, Ireland.
• Jul. 2012 – Sep. 2013. Postdoctoral Research Fellow in Dr. Tsai-Ling Yang Lauderdale’ s Lab. National Institute of Infectious Diseases and Vaccinology, National Health Research Institute, Taiwan.

ORCID ID https://orcid.org/0000-0002-7676-0239

-In Meso In Situ Serial X-Ray Crystallography (IMISX) and Sample Delivery

I. Martiel, C. Padeste, A. Karpik, J. H. Beale, C.-Y. Huang, L. Vera, N. Olieric, M. Wranik, C.-J. Tsai, J. Muhle, O. Aurelius, J. John, M. Hogbom, M. Wang and M. Marsh. Versatile microporous polymer-based supports for serial macromolecular crystallography. 2021. Acta Cryst. D77. 1153-1167. https://doi.org/10.1107/S2059798321007324

I. Martiel, C.-Y. Huang, P. Villanueva-Perez, E. Panepucci, B. Basu, M. Caffrey, B. Pedrini, O. Bunk, M. Stampanoni & M. Wang. Low-dose in situ prelocation of protein microcrystals by 2D X-ray phase-contrast imaging for serial crystallography. 2020. IUCrJ. 7(6):1131-1141. https://doi.org/10.1107/S2052252520013238

C.-Y. Huang, N.A. Meier, M. Caffrey, M. Wang & V. Olieric. 3D-printed holders for in meso in situ fixed-target serial X-ray crystallography. Journal of Applied Crystallography. 2020; 53(3): https://doi.org/10.1107/S1600576720002897

C.-Y. Huang, V. Olieric, M. Caffrey & M. Wang. In meso in situ serial X-Ray crystallography (IMISX): a protocol for membrane protein structure determination at the Swiss Light Source In: Perez C, Maier T, eds. Expression, purification, and structural biology of membrane proteins. Methods in molecular biology. New York: Humana; 2020. https://doi.org/10.1007/978-1-0716-0373-4_20

R. Cheng, C.-Y. Huang, M. Hennig, H. Nar & G. Schnapp. In situ crystallography as an emerging method for structure solution of membrane proteins: the case of CCR2A FEBS Journal. 2020; 287(5): 866-873. https://doi.org/10.1111/febs.15098

A.K. Apel, R.K.Y. Cheng, C.S. Tautermann, M. Brauchle, C.-Y. Huang, A. Pautsch, M. Hennig, H. Nar & G. Schnapp. Crystal structure of CC chemokine receptor 2A in complex with an orthosteric antagonist provides insights for the design of selective antagonists Structure. 2019; 27(3): 427-438.e5. https://doi.org/10.1016/j.str.2018.10.027

M. El Ghachi, N. Howe, C.-Y. Huang, V. Olieric, R. Warshamanage, T. Touzé, D. Weichert, P.J. Stansfeld, M. Wang, F. Kerff & M. Caffrey. Crystal structure of undecaprenyl-pyrophosphate phosphatase and its role in peptidoglycan biosynthesis Nature Communications. 2018; 9(1): 1078 (13 pp.). https://doi.org/10.1038/s41467-018-03477-5

C.-Y. Huang, V. Olieric, N. Howe, R. Warshamanage, T. Weinert, E. Panepucci, L. Vogeley, S. Basu, K. Diederichs, M. Caffrey & M. Wang. In situ serial crystallography for rapid de novo membrane protein structure determination. Communications Biology. 2018; 1: 124 (8 pp.). https://doi.org/10.1038/S42003-018-0123-6

C.-Y. Huang, V. Olieric, P. Ma, N. Howe, L. Vogeley, X. Liu, R. Warshamanage, T. Weinert, E. Panepucci, B. Kobilka, K. Diederichs, M. Wang & M. Caffrey. In meso in situ serial X-ray crystallography of soluble and membrane proteins at cryogenic temperatures Acta Crystallographica Section D: Structural Biology. 2016; 72(1): 93-112. https://doi.org/10.1107/S2059798315021683

C.-Y. Huang, V. Olieric, P. Ma, E. Panepucci, K. Diederichs, M. Wang & M. Caffrey. In meso in situ serial X-ray crystallography of soluble and membrane proteins Acta Crystallographica Section D: Structural Biology. 2015; 71(6): 1238-1256. https://doi.org/10.1107/S1399004715005210

-Membrane Protein Structural Biology

L. Smithers^#, O. Degtjarik^#, D. Weichert^#, C.-Y. Huang^#, C. Boland, K. Bowen, A. Oluwole, C. Lutomski, C. V. Robinson, E. M. Scanlan, M. Wang, V. Olieric, M. Shalev-Benami & M. Caffrey. Structure snapshots reveal the mechanism of a bacterial membrane lipoprotein N-acyltransferase. Sci. Adv. 2023. 9:(26): eadf5799. htpps://doi.org/10.1126/sciadv.adf5799 ^#Contributed equally to this paper.

H. T. V. Nguyen, X. Chen, C. Parada, A. C. Luo, O. Shih, U. S. Jeng, C.-Y. Huang & C. Ma. Structure of the heterotrimeric membrane protein complex FtsB-FtsL-FtsQ of the bacterial divisome. Nature Communications. 2023. 14(1), 1903 (13 pp.). https://doi.org/10.1038/s41467-023-37543-4

H. Wang, C. Mörman, R. Sternke-Hoffmann, C.-Y. Huang, A. Prota, P. Ma & J. Luo. Cu 2+ ions modulate the interaction between α-synuclein and lipid membranes. J. Inorg. Biochem. 2022. 236:111945. https://doi.org/10.1016/j.jinorgbio.2022.111945

Q. Xu, N. Sun, Q. Xiao, C.-Y. Huang, M. Xu, W. Zhang, L. Li, Q. Wang, V. Olieric, W. Wang, J. He & B. Sun. The crystal structure of MreC provides insights into polymer formation. 2022. FEBS Open Bio. 12(2):340-348. https://doi.org/10.1002/2211-5463.13296

S. Olatunji, K. Bowen, C.-Y. Huang, D. Weichert, W. Singh, I. Tikhonova, E. M. Scanlan, V. Olieric & M. Caffrey. Structural basis of the membrane intramolecular transacylase reaction responsible for lyso-form lipoprotein synthesis. 2021. Nat. Commun. 12:4254. https://doi.org/10.1038/s41467-021-24475-0

C. Nasrallah, G. Cannone, J. Briot, K. Rottier, A. E. Berizzi, C.-Y. Huang, R. B. Quast, F. Hoh, J.-L. Banères, F. Malhaire, L. Berto, A. Dumazer, J. Font-Ingles, X. Gómez-Santacana, J. Catena, J. Kniazeff, C. Goudet, A. Llebaria, J.-P. Pin, K. R. Vinothkumar & G. Lebon. Agonists and allosteric modulators promote signaling from different metabotropic glutamate receptor 5 conformations. 2021. Cell Rep. 36 (9):109648. https://doi.org/10.1016/j.celrep.2021.109648

S. Olatunji, X. Yu, J. Bailey, C.-Y. Huang, M. Zapotoczna, K. Bowen, M. Remškar, R. Müller, E.M. Scanlan, J.A. Geoghegan, V. Olieric & M. Caffrey. Structures of lipoprotein signal peptidase II from Staphylococcus aureus complexed with antibiotics globomycin and myxovirescin Nature Communications. 2020; 11(1): 140 (11 pp.). https://doi.org/10.1038/s41467-019-13724-y

J.C. Crack, P. Amara, A. Volbeda, J.M. Mouesca, R. Rohac, M.T. P. Martinez, C.-Y. Huang, O. Gigarel, C. Rinaldi, N.E. Le Brun & J.C. Electron and proton transfers modulate DNA binding by the transcription regulator RsrR Journal of the American Chemical Society. 2020; 142(11): 5104-5116. https://doi.org/10.1021/jacs.9b12250

H. Li^#, C.-Y. Huang^#, E. G. Govorunova, O. A. Sineshchekov, M. Wang, L. Zheng & J. L. Spudich. The Crystal Structure of Bromide-Bound GtACR1 Reveals a Pre-Activated State in the Transmembrane Anion Tunnel. Elife. 2021. eLife. 10: e65903. https://doi.org/10.7554/eLife.65903 ^#Contributed equally to this paper.

H. Li, C.-Y. Huang, E.G. Govorunova, C.T. Schafer, O.A. Sineshchekov, M. Wang, L. Zheng & J.L. Spudich. Crystal structure of a natural light-gated anion channelrhodopsin. 2019. eLife. 8: e41741. https://doi.org/10.7554/eLife.41741

K. Jaeger, S. Bruenle, T. Weinert, W. Guba, J. Muehle, Y. Miyazaki, M .Weber, A. Furrer, N. Haenggi, T. Tetaz, C.-Y. Huang, D. Mattle, J.M. Vonach, A. Gast, A. Kuglstatter, M.G. Rudolph, P. Nogly, J. Benz & R.J.P. Dawson, J. Standfuss. Structural Basis for Allosteric Ligand Recognition in the Human CC Chemokine Receptor 7. 2019. Cell. 178: 1222-1230.e10. https://doi.org/10.1016/j.cell.2019.07.028

W. Yin, X.E. Zhou, D. Yang, P.W. de Waal, M. Wang, A. Dai, X. Cai, C.-Y. Huang, P. Liu, X. Wang, Y. Yin, B. Liu, Y. Zhou, J. Wang, H. Liu, M. Caffrey, K. Melcher, Y. Xu, M. Wang, H.E. Xu & Y. Jiang. Crystal structure of the human 5-HT1B serotonin receptor bound to an inverse agonist Cell Discovery. 2018; 4: 12 (13 pp.). https://doi.org/10.1038/s41421-018-0009-2

M. Wiktor, D. Weichert, N. Howe, C.-Y. Huang, V. Olieric, C. Boland, J. Bailey, L. Vogeley, P.J. Stansfeld, N. Buddelmeijer, M. Wang & M. Caffrey. Structural insights into the mechanism of the membrane integral N-acyltransferase step in bacterial lipoprotein synthesis Nature Communications. 2017; 8: 15952 (13 pp.). https://doi.org/10.1038/ncomms15952

-Room Temperature X-ray Crystallography

C.-Y. Huang^#, S. Aumonier^#, S. Engilberge^#, D. Eris, K. M. L. Smith, F. Leonarski, J. A. Wojdyla, J. H. Beale, D. Buntschu, A. Pauluhn, M. E. Sharpe, A. Metz, V. Olieric & M. Wang. Probing ligand binding of endothiapepsin by `temperature-resolved' macromolecular crystallography. 2022. Acta Cryst. D78: 964-974. https://doi.org/10.1107/S205979832200612X ^#Contributed equally to this paper.

-Long-Wavelength Structural Phasing

Basu S, Olieric V, Leonarski F, Matsugaki N, Kawano Y, Takashi T, C.-Y. Huang, Y. Yamada, L. Vera, N. Olieric, J. Basquin, J. A. Wojdyla, O. Bunk, K. Diederichs, M. Yamamoto & M. Wang Long-wavelength native-SAD phasing: opportunities and challenges IUCrJ. 2019; 6(3): 1-14. https://doi.org/10.1107/S2052252519002756

-Beamline Software and Automation

 K. M. L. Smith, E. Panepucci, J. W. Kaminski, S. Aumonier, C. Y. Huang, D. Eris, D. Buntschu, N. Meier, W. Glettig, K.E. McAuley, M. Wang, M.E. Sharpe & J. A. Wojdyla. SDU - software for high-throughput automated data collection at the Swiss Light Source. Journal of Synchrotron Radiation. 2023. 30: 538-545. https://doi.org/10.1107/S1600577523002631

J. W. Kaminski, L. Vera, D. P. Stegmann, J. Vering, D. Eris, K. M. L. Smith, C.-Y. Huang, N. Meier, J. Steuber, M. Wang, G. Fritz, J. A. Wojdyla & M. E. Sharpe. 2022. Fast fragment- and compound-screening pipeline at the Swiss Light Source. Acta Cryst. D78: 328-336. https://doi.org/10.1107/S2059798322000705

S. Basu, J.W. Kaminski, E .Panepucci, C.-Y. Huang, R. Warshamanage, M. Wang & J.A. Wojdyla. Automated data collection and real-time data analysis suite for serial synchrotron crystallography Journal of Synchrotron Radiation. 2019; 26(1): 244-252. https://doi.org/10.1107/S1600577518016570

J.A. Wojdyla, E. Panepucci, I. Martiel, S. Ebner, C.-Y. Huang, M. Caffrey, O. Bunk & M. Wang. Fast two-dimensional grid and transmission X-ray microscopy scanning methods for visualizing and characterizing protein crystals Journal of Applied Crystallography. 2016; 49: 944-952. https://doi.org/10.1107/S1600576716006233