TOMCAT - X02DA: Tomographic MicroscopyA beamline for TOmographic Microscopy and Coherent rAdiology experimenTs
The beamline for TOmographic Microscopy and Coherent rAdiology experimentTs (TOMCAT)  offers cutting-edge technology and scientific expertise for exploiting the distinctive peculiarities of synchrotron radiation for fast, nondestructive, high resolution, quantitative investigations on a large variety of samples. Absorption-based and phase contrast imaging are routinely performed with isotropic voxel sizes ranging from 0.16 to 6.5 μm (fields-of-view of 0.4 x 0.3 mm2 and 16.6 x 14.0 mm2, respectively) in an energy range of 8-45 keV. Phase contrast is obtained with simple edge-enhanced, propagation-based techniques [2,3] or through grating interferometry .
Typical acquisition times are on the order of seconds to a few minutes. However, dynamic processes can be followed in 4D (3D space + time) using the ultra-fast endstation, which provides sub-second temporal resolution . A laser-based heating system  and a cryojet and cryo-chamber are available as standard installations and are compatible with both the standard and ultra-fast endstations. A sample exchanger and a package of automation tools  are accessible for performing high-throughput studies in a fully automatic manner. It is also possible to bring specialized, user-defined instrumentation to TOMCAT. Please contact beamline staff in advance to discuss this option.
3D tomographic datasets are reconstructed from 2D projections using highly optimized software [8-10] based on Fourier methods and a user-friendly interface (i.e., an ImageJ plug-in). A fully automated package for the quantitative analysis (segmentation, labeling, quantitative morphological characterization, tensor analysis) of cellular materials is available on a collaborative basis .
|Energy range||8-45 keV|
|Highest 3D spatial resolution|| ca. 1 μm in parallel beam geometry
ca. 200 nm in full-field geometry
|Max. temporal resolution||10 Hz|
|Available techniques|| - Absorption-based tomographic microscopy
- Propagation-based phase contrast tomographic microscopy
- Ultra-fast tomographic microscopy
- Differential phase contrast (DPC) tomographic microscopy
- Absorption and phase contrast nanotomography
|Available devices for in situ sample conditioning|| - Laser-based heating system
- Cryojet and cryo-chamber
|Photon source divergence (tailored by aperture)||2mrad, 0.6 mrad|
- M. Stampanoni, A. Groso, A. Isenegger, G. Mikuljan, Q. Chen, A. Bertrand, S. Henein, R. Betemps, U. Frommherz, P. Bohler, D. Meister, M. Lange, and R. Abela, Trends in synchrotron-based tomographic imaging: the SLS experience, _Developments in X-Ray Tomography V, Proceedings of the Society of Photo-Optical Instrumentation Engineers (Spie)_, 6318, U199-U212 (2006).
- A. Groso, R. Abela, and M. Stampanoni, Implementation of a fast method for high resolution phase contrast tomography, Optics Express, 14, 8103-8110 (2006). DOI: 10.1364/OE.14.008103
- D. Paganin, S. C. Mayo, T. E. Gureyev, P. R. Miller, and S. W. Wilkins, Simultaneous phase and amplitude extraction from a single defocused image of a homogeneous object, Journal of Microscopy, 206, 33-40 (2002). DOI: 10.1046/j.1365-2818.2002.01010.x
- S. A. McDonald, F. Marone, C. Hintermüller, G. Mikuljan, C. David, F. Pfeiffer, and M. Stampanoni, Advanced phase-contrast imaging using a grating interferometer, J. Synchrotron Rad., 16, 562-572 (2009). DOI: 10.1107/S0909049509017920
- R. Mokso, F. Marone, D. Haberthur, J. C. Schittny, G. Mikuljan, A. Isenegger, and M. Stampanoni, Following Dynamic Processes by X-ray Tomographic Microscopy with Sub-second Temporal Resolution, 10th International Conference on X-Ray Microscopy, 1365, 38-41 (2011). DOI: 10.1063/1.3625299
- J. L. Fife, M. Rappaz, M. Pistone, T. Celcer, G. Mikuljan, and M. Stampanoni, Development of a laser-based heating system for in-situ synchrotron-based x-ray tomographic microscopy, J. Synchrotron Rad., 19, 352 (2012). DOI: 10.1107/S0909049512003287
- K. Mader, F. Marone, C. Hintermuller, G. Mikuljan, A. Isenegger, and M. Stampanoni, High-throughput full-automatic synchrotron-based tomographic microscopy, J. Synchrotron Rad., 18, 117-124 (2011). DOI: 10.1107/S0909049510047370
- C. Hintermuller, F. Marone, A. Isenegger, and M. Stampanoni, Image processing pipeline for synchrotron-radiation-based tomographic microscopy, J. Synchrotron Rad., 17, 550-559 (2010). DOI: 10.1107/S0909049510011830
- F. Marone, B. Munch, and M. Stampanoni, Fast reconstruction algorithm dealing with tomography artifacts, Developments in X-Ray Tomography Vii, Proceedings of SPIE-The International Society for Optical Engineering, 7804 (2010). DOI: 10.1117/12.859703
- F. Marone, and M. Stampanoni, Regridding reconstruction algorithm for real time tomographic imaging, J. Synchrotron Rad., 19, 1029-1037 (2012). DOI: 10.1107/S0909049512032864
- K. Mader, R. Mokso, C. Raufaste, B. Dollet, S. Santucci, J. Lambert, and M. Stampanoni, Quantitative 3D characterization of cellular materials: Segmentation and morphology of foam, Colloids and Surfaces A: Physicochemical and Engineering Aspects, 415, 230-238 (2012). DOI: 10.1016/j.colsurfa.2012.09.007