Dr. Florian Dworkowski

photo of Florian Dworkowski

Beamline Scientist

Paul Scherrer Institute
Forschungsstrasse 111
5232 Villigen PSI

  • Beamline Instrumentation
  • Serial Crystallography
  • New Method and Software Development
  • in situ Spectroscopy
  • Radiation Damage
  • since 2013 - Beamline Scientist in the Macromolecular Crystallography Group, Swiss Light Source, Switzerland
  • 2010-2012 - PostDoc in the Macromolecular Crystallography Group, Swiss Light Source, Switzerland
  • 2005-2009 - Ph.D. in Biology & Biophysics, Institute for Molecular Biology and Biophysics, Swiss Federal Institute of Technology (ETH), Switzerland
  • 2002-2004 - M.Sc. in Chemistry and Biochemistry, Department for Chemistry and Biochemistry, The University of Oklahoma, USA
  • 2000-2002 - in Chemistry, Department for Chemistry, Technische Universität Berlin, Germany
  • Lučić M, Chaplin AK, Moreno-Chicano T, Dworkowski FSN, Wilson MT, Svistunenko DA, et al.
    A subtle structural change in the distal haem pocket has a remarkable effect on tuning hydrogen peroxide reactivity in dye decolourising peroxidases from Streptomyces lividans
    Dalton Transactions. 2020; 49(5): 1620-1636. https://doi.org/10.1039/c9dt04583j
  • Lučić M, Svistunenko DA, Wilson MT, Chaplin AK, Davy B, Ebrahim A, et al.
    Serial femtosecond zero dose crystallography captures a water-free distal heme site in a dye‐decolourising peroxidase to reveal a catalytic role for an arginine in FeIV=O formation
    Angewandte Chemie International Edition. 2020; 59(48): 21656-21662. https://doi.org/10.1002/anie.202008622
  • Nass K, Cheng R, Vera L, Mozzanica A, Redford S, Ozerov D, et al.
    Advances in long-wavelength native phasing at X-ray free-electron lasers
    IUCrJ. 2020; 7: 965-975. https://doi.org/10.1107/S2052252520011379
  • Skopintsev P, Ehrenberg D, Weinert T, James D, Kar RK, Johnson PJM, et al.
    Femtosecond-to-millisecond structural changes in a light-driven sodium pump
    Nature. 2020; 583: 314-318. https://doi.org/10.1038/s41586-020-2307-8
  • Weinert T, Skopintsev P, James D, Dworkowski F, Panepucci E, Kekilli D, et al.
    Proton uptake mechanism in bacteriorhodopsin captured by serial synchrotron crystallography
    Science. 2019; 365(6448): 61-65. https://doi.org/10.1126/science.aaw8634
  • Horrell S, Kekilli D, Sen K, Owen RL, Dworkowski FSN, Antonyuk SV, et al.
    Enzyme catalysis captured using multiple structures from one crystal at varying temperatures
    IUCrJ. 2018; 5(3): 283-292. https://doi.org/10.1107/S205225251800386X
  • Lebugle M, Dworkowski F, Pauluhn A, Guzenko VA, Romano L, Meier N, et al.
    High-intensity x-ray microbeam for macromolecular crystallography using silicon kinoform diffractive lenses
    Applied Optics. 2018; 57(30): 9032-9039. https://doi.org/10.1364/AO.57.009032
  • Kekilli D, Petersen CA, Pixton DA, Ghafoor DD, Abdullah GH, Dworkowski FSN, et al.
    Engineering proximal vs. distal heme-NO coordination via dinitrosyl dynamics: implications for NO sensor design
    Chemical Science. 2017; 8(3): 1986-1994. https://doi.org/10.1039/c6sc04190f
  • Kekilli D, Moreno-Chicano T, Chaplin AK, Horrell S, Dworkowski FSN, Worrall JAR, et al.
    Photoreduction and validation of haem-ligand intermediate states in protein crystals by in situ single-crystal spectroscopy and diffraction
    IUCrJ. 2017; 4: 263-270. https://doi.org/10.1107/S2052252517002159
  • Weinert T, Olieric N, Cheng R, Brünle S, James D, Ozerov D, et al.
    Serial millisecond crystallography for routine room-temperature structure determination at synchrotrons
    Nature Communications. 2017; 8(1): 542 (11 pp.). https://doi.org/10.1038/s41467-017-00630-4
  • Jaeger K, Dworkowski F, Nogly P, Milne C, Wang M, Standfuss J
    Serial millisecond crystallography of membrane proteins
    In: Moraes I, ed. The next generation in membrane protein structure determination. Advances in experimental medicine and biology. Cham: Springer; 2016:137-149. https://doi.org/10.1007/978-3-319-35072-1_10
  • Botha S, Nass K, Barends T, Kabsch W, Latz B, Dworkowski F, et al.
    Room-temperature serial crystallography at synchrotron X-ray sources using slowly flowing free-standing high-viscosity microstreams
    Acta Crystallographica Section D: Structural Biology. 2015; 71: 387-397. https://doi.org/10.1107/S1399004714026327
  • Dworkowski FSN, Hough MA, Pompidor G, Fuchs MR
    Challenges and solutions for the analysis of in situ, in crystallo micro-spectrophotometric data
    Acta Crystallographica Section D: Structural Biology. 2015; 71: 27-35. https://doi.org/10.1107/S1399004714015107
  • Ghafoor DD, Kekilli D, Abdullah GH, Dworkowski FSN, Hassan HG, Wilson MT, et al.
    Hydrogen bonding of the dissociated histidine ligand is not required for formation of a proximal NO adduct in cytochrome c'
    Journal of Biological Inorganic Chemistry. 2015; 20(6): 949-956. https://doi.org/10.1007/s00775-015-1278-y
  • Angelaccio S, Dworkowski F, Di Bello A, Milano T, Capitani G, Pascarella S
    Conformational transitions driven by pyridoxal-5′-phosphate uptake in the psychrophilic serine hydroxymethyltransferase from Psychromonas ingrahamii
    Proteins. 2014; 82(10): 2831-2841. https://doi.org/10.1002/prot.24646
  • Bolze CS, Helbling RE, Owen RL, Pearson AR, Pompidor G, Dworkowski F, et al.
    Human cellular retinaldehyde-binding protein has secondary thermal 9-cis-retinal isomerase activity
    Journal of the American Chemical Society. 2014; 136(1): 137-146. https://doi.org/10.1021/ja411366w
  • Fuchs MR, Pradervand C, Thominet V, Schneider R, Panepucci E, Grunder M, et al.
    D3, the new diffractometer for the macromolecular crystallography beamlines of the Swiss Light Source
    Journal of Synchrotron Radiation. 2014; 21(2): 340-351. https://doi.org/10.1107/S160057751400006X
  • Kekilli D, Dworkowski FSN, Pompidor G, Fuchs MR, Andrew CR, Antonyuk S, et al.
    Fingerprinting redox and ligand states in haemprotein crystal structures using resonance Raman spectroscopy
    Acta Crystallographica Section D: Structural Biology. 2014; 70(5): 1289-1296. https://doi.org/10.1107/S1399004714004039
  • Merlino A, Fuchs MR, Pica A, Balsamo A, Dworkowski FSN, Pompidor G, et al.
    Selective X-ray-induced NO photodissociation in haemoglobin crystals: evidence from a Raman-assisted crystallographic study
    Acta Crystallographica Section D: Structural Biology. 2013; 69(1): 137-140. https://doi.org/10.1107/S0907444912042229
  • Pompidor G, Dworkowski FSN, Thominet V, Schulze-Briese C, Fuchs MR
    A new on-axis micro-spectrophotometer for combining Raman, fluorescence and UV/Vis absorption spectroscopy with macromolecular crystallography at the Swiss Light Source
    Journal of Synchrotron Radiation. 2013; 20(5): 765-776. https://doi.org/10.1107/S0909049513016063
  • Rajendran C, Dworkowski FSN, Wang M, Schulze-Briese C
    Radiation damage in room-temperature data acquisition with the PILATUS 6M pixel detector
    Journal of Synchrotron Radiation. 2011; 18(3): 318-328. https://doi.org/10.1107/S090904951100968X
  • Eidam O, Dworkowski FSN, Glockshuber R, Grütter MG, Capitani G
    Crystal structure of the ternary FimC-FimFt-FimDN complex indicates conserved pilus chaperone-subunit complex recognition by the usher FimD
    FEBS Letters. 2008; 582(5): 651-655. https://doi.org/10.1016/j.febslet.2008.01.030