Dr. Florian Dworkowski

photo of Florian Dworkowski

Beamline Scientist

Paul Scherrer Institute
Forschungsstrasse 111
5232 Villigen PSI
Switzerland

  • Beamline Instrumentation
  • Serial Crystallography
  • New Method and Software Development
  • in situ Spectroscopy
  • Radiation Damage
  • since 2013 - Beamline Scientist in the Macromolecular Crystallography Group, Swiss Light Source, Switzerland
  • 2010-2012 - PostDoc in the Macromolecular Crystallography Group, Swiss Light Source, Switzerland
  • 2005-2009 - Ph.D. in Biology & Biophysics, Institute for Molecular Biology and Biophysics, Swiss Federal Institute of Technology (ETH), Switzerland
  • 2002-2004 - M.Sc. in Chemistry and Biochemistry, Department for Chemistry and Biochemistry, The University of Oklahoma, USA
  • 2000-2002 - in Chemistry, Department for Chemistry, Technische Universität Berlin, Germany
  • Weinert T, Skopintsev P, James D, Dworkowski F, Panepucci E, Kekilli D, et al.
    Proton uptake mechanism in bacteriorhodopsin captured by serial synchrotron crystallography
    Science. 2019; 365(6448): 61-65. https://doi.org/10.1126/science.aaw8634
    DORA PSI
  • Horrell S, Kekilli D, Sen K, Owen RL, Dworkowski FSN, Antonyuk SV, et al.
    Enzyme catalysis captured using multiple structures from one crystal at varying temperatures
    IUCrJ. 2018; 5: 283-292. https://doi.org/10.1107/S205225251800386X
    DORA PSI
  • Lebugle M, Dworkowski F, Pauluhn A, Guzenko VA, Romano L, Meier N, et al.
    High-intensity x-ray microbeam for macromolecular crystallography using silicon kinoform diffractive lenses
    Applied Optics. 2018; 57(30): 9032-9039. https://doi.org/10.1364/AO.57.009032
    DORA PSI
  • Kekilli D, Petersen CA, Pixton DA, Ghafoor DD, Abdullah GH, Dworkowski FSN, et al.
    Engineering proximal vs. distal heme-NO coordination via dinitrosyl dynamics: implications for NO sensor design
    Chemical Science. 2017; 8(3): 1986-1994. https://doi.org/10.1039/c6sc04190f
    DORA PSI
  • Kekilli D, Moreno-Chicano T, Chaplin AK, Horrell S, Dworkowski FSN, Worrall JAR, et al.
    Photoreduction and validation of haem-ligand intermediate states in protein crystals by in situ single-crystal spectroscopy and diffraction
    IUCrJ. 2017; 4: 263-270. https://doi.org/10.1107/S2052252517002159
    DORA PSI
  • Weinert T, Olieric N, Cheng R, Brünle S, James D, Ozerov D, et al.
    Serial millisecond crystallography for routine room-temperature structure determination at synchrotrons
    Nature Communications. 2017; 8(1): 542 (11 pp.). https://doi.org/10.1038/s41467-017-00630-4
    DORA PSI
  • Jaeger K, Dworkowski F, Nogly P, Milne C, Wang M, Standfuss J
    Serial millisecond crystallography of membrane proteins
    In: Moraes I, ed. The next generation in membrane protein structure determination. Advances in experimental medicine and biology. Cham: Springer; 2016. https://doi.org/10.1007/978-3-319-35072-1_10
    DORA PSI
  • Botha S, Nass K, Barends T, Kabsch W, Latz B, Dworkowski F, et al.
    Room-temperature serial crystallography at synchrotron X-ray sources using slowly flowing free-standing high-viscosity microstreams
    Acta Crystallographica Section D: Structural Biology. 2015; 71: 387-397. https://doi.org/10.1107/S1399004714026327
    DORA PSI
  • Dworkowski FSN, Hough MA, Pompidor G, Fuchs MR
    Challenges and solutions for the analysis of in situ, in crystallo micro-spectrophotometric data
    Acta Crystallographica Section D: Structural Biology. 2015; 71: 27-35. https://doi.org/10.1107/S1399004714015107
    DORA PSI
  • Ghafoor DD, Kekilli D, Abdullah GH, Dworkowski FSN, Hassan HG, Wilson MT, et al.
    Hydrogen bonding of the dissociated histidine ligand is not required for formation of a proximal NO adduct in cytochrome c'
    Journal of Biological Inorganic Chemistry. 2015; 20(6): 949-956. https://doi.org/10.1007/s00775-015-1278-y
    DORA PSI
  • Angelaccio S, Dworkowski F, Di Bello A, Milano T, Capitani G, Pascarella S
    Conformational transitions driven by pyridoxal-5′-phosphate uptake in the psychrophilic serine hydroxymethyltransferase from Psychromonas ingrahamii
    Proteins. 2014; 82(10): 2831-2841. https://doi.org/10.1002/prot.24646
    DORA PSI
  • Bolze CS, Helbling RE, Owen RL, Pearson AR, Pompidor G, Dworkowski F, et al.
    Human cellular retinaldehyde-binding protein has secondary thermal 9-cis-retinal isomerase activity
    Journal of the American Chemical Society. 2014; 136(1): 137-146. https://doi.org/10.1021/ja411366w
    DORA PSI
  • Fuchs MR, Pradervand C, Thominet V, Schneider R, Panepucci E, Grunder M, et al.
    D3, the new diffractometer for the macromolecular crystallography beamlines of the Swiss Light Source
    Journal of Synchrotron Radiation. 2014; 21(2): 340-351. https://doi.org/10.1107/S160057751400006X
    DORA PSI
  • Kekilli D, Dworkowski FSN, Pompidor G, Fuchs MR, Andrew CR, Antonyuk S, et al.
    Fingerprinting redox and ligand states in haemprotein crystal structures using resonance Raman spectroscopy
    Acta Crystallographica Section D: Structural Biology. 2014; 70(5): 1289-1296. https://doi.org/10.1107/S1399004714004039
    DORA PSI
  • Merlino A, Fuchs MR, Pica A, Balsamo A, Dworkowski FSN, Pompidor G, et al.
    Selective X-ray-induced NO photodissociation in haemoglobin crystals: Evidence from a Raman-assisted crystallographic study
    Acta Crystallographica Section D: Structural Biology. 2013; 69(1): 137-140. https://doi.org/10.1107/S0907444912042229
    DORA PSI
  • Pompidor G, Dworkowski FSN, Thominet V, Schulze-Briese C, Fuchs MR
    A new on-axis micro-spectrophotometer for combining Raman, fluorescence and UV/Vis absorption spectroscopy with macromolecular crystallography at the Swiss Light Source
    Journal of Synchrotron Radiation. 2013; 20(5): 765-776. https://doi.org/10.1107/S0909049513016063
    DORA PSI
  • Rajendran C, Dworkowski FSN, Wang M, Schulze-Briese C
    Radiation damage in room-temperature data acquisition with the PILATUS 6M pixel detector
    Journal of Synchrotron Radiation. 2011; 18(3): 318-328. https://doi.org/10.1107/S090904951100968X
    DORA PSI
  • Eidam O, Dworkowski FSN, Glockshuber R, Grütter MG, Capitani G
    Crystal structure of the ternary FimC-FimFt-FimDN complex indicates conserved pilus chaperone-subunit complex recognition by the usher FimD
    FEBS Letters. 2008; 582(5): 651-655. https://doi.org/10.1016/j.febslet.2008.01.030
    DORA PSI