Electrophysiological methods and devicesfor the activity measurement of membrane proteins
For more information, contact Dr. Jinghui Luo
- Two instruments (Potentiostat Autolab, PG12) are available, equipped with an impedance and a low current module. Voltage-clamp measurements are carried out using an Axopatch 200 B instrument and a Digidata 1440 A analyser inside a Faraday cage (Warner) .
- Rotavap and extruders are used to generate liposomes of uniform sizes.
- Transport of solvents in microfluidic systems is achieved by computer-controlled NEMESYS syringe devices.
Laboratory for micro- and nanotechnolgy and external partners.
Two kinds of materials have been used for bioanalytical devices: silicon and polymers. Silicon technology requires clean room laboratories including essentially photolithography or e-beam facilities for structure definition and etching processes for structure generation. Polymers can be structured by hot embossing and molding techniques and surfaces can be functionalized using chemical methods. Scanning electron microscopy, contact angle measurements and fluorescence microscopy are methods to analyse surface properties.
The quality of bilayer sealing depends on the lipid composition, the bilayer formation method, the material and the surface properties (roughness, contact angle). The capacitance mainly depends on the thickness of the supporting material. If it is thinner than about 10 micrometers, the capacitance values can be hardly compensated to the low values necessary for Voltage-clamp experiments.