Dr. Ana Diaz

photo of Ana Diaz

Beamline Scientist

Paul Scherrer Institute
Forschungsstrasse 111
5232 Villigen PSI

Ana Diaz has dedicated her entire career to develop X-ray characterization methods using synchrotron radiation. She did her PhD at the Paul Scherrer Institute in Switzerland on the characterization of colloidal suspensions confined in unidimensional gratings and then moved to the European Synchrotron Radiation Facility in France to work as a postdoc, where she used Bragg coherent diffraction imaging to characterize epitaxial SiGe nanocrystalline structures. She is now back at the Paul Scherrer Institute where she works as a beamline scientist at the Swiss Light Source since 2009. Ana has contributed significantly to the implementation of hard X-ray ptychography at the cSAXS beamline, in particular for ptychographic tomography. Together with her colleagues, she has received the Helmoltz Zentrum Berlin Innovation Award on Synchrotron Radiation in 2014 for high-resolution 3D hard X-ray microscopy.

Commissioning and further development of experimental setups at the coherent small-angle X-ray scattering (cSAXS) beamline at the Swiss Light Source (SLS), research using synchrotron radiation, publication of results in specialized journals and presentation in conferences, user support at the cSAXS beamline at the SLS, and support of master students, PhD students and postdocs.

Ana Diaz's main research interest is the application of x-ray ptychographic imaging for systems which can not be easily investigated with other microscopy techniques. Examples of such sytems are cryo x-ray microscopy of biological tissue and imaging of strain fields in nanocrystalline materials.

For an extensive overview we kindly refer you to our publication repository DORA and to Ana Diaz's researcher ID I-4139-2013

X-ray Fourier ptychography, K. Wakonig, A. Diaz, A. Bonnin, M. Stampanoni, A. Bergamaschi, J. Ihli, M. Guizar-Sicairos, A. Menzel, Sci. Adv. 5, eaav0282 (2019) DOI: 10.1126/sciadv.aav0282  First demonstration of Fourier ptychography using X-rays. This work shows the way to enhance existing lens-based X-ray microscopes to obtain quantitative phase contrast with spatial resolution beyond that limited by the lens.

Evolutionary‐Optimized Photonic Network Structure in White Beetle Wing Scales, B. D, Wilts, X. Sheng, M. Holler, A. Diaz, M. Guizar-Sicairos, J. Raabe, R. Hoppe, S.-H. Liu, R. Langford, O. D. Onelli, D. Chen, S. Torquato, U. Steiner, C. G. Schroer, S. Vognolini, A. Sepe, Adv. Mater. 30, 1702057 (2018) DOI: 10.1002/adma.201702057 Non-destructive imaging of an intact part of a beetle's scale revealed its 3D photonic structure with a spatial resolution of 30 nm. Simulations were then performed on the imaged structure to confirm its evolutionary optimization: the structure possesses the minimum amount of material to obtain optimal light reflectivity, which gives the insect a white color while keeping an efficient flying capability. The images were produced using the OMNY instrument developed at the Swiss Light Source, featuring a cryo-stage with scanning accuracy on the nanoscale. In previous studies, electron microscopy images required the acquisition of 2D images followed by the removal of a thin layer of the material in consecutive steps. The delicate structure was not withstanding this destructive approach and therefore an image of the intact 3D structure was not possible.

A three-dimensional view of structural changes caused by deactivation of fluid catalytic cracking catalysts, J. Ihli, R. R. Jacob, M. Holler, M. Guizar_Sicairos, A. Diaz, J. C. da Silva, D. Ferreira Sanchez, F. Krumeich, D. Grolimund, M. Taddei, W.-C. Cheng, Y. Shu, A. Menzel, J. A. van Bokhoven, Nat. Commun. 8, 809 (2017), DOI: 10.1038/s41467-017-00789-w This structural study of fluid catalytic cracking particles reveals a mechanism of deactivation of these particles caused by an amorphization of zeolites close to the particle exterior, which results in a reduction of catalytic activity.

Three-dimensional imaging of biological tissue by cryo X-ray ptychography, S. H. Shahmoradian, E. H. R. Tsai, A. Diaz, M. Guizar-Sicairos, J. Raabe, L. Spycher, M. Britschgi, A. Ruf, H. Stahlberg, M. Holler, Sci. Rep. 7, 6291 (2017), DOI: 10.1038/s41598-017-05587-4 First demonstration of non-destructive 3D imaging of frozen hydrated brain tissue. The relevance of this works lies in the possibility to visualize a large volume of brain tissue of several tens of microns in each dimension while enabling further inspection of smaller areas with other established electron microscopy techniques that require the specimen to remain in a hydrated state. The images were acquired with OMNY, a unique instrument available at the Swiss Light Source.

Three-dimensional mass density mapping of cellular ultrastructure by ptychographic X-ray nanotomography, A. Diaz, B. Malkova, M. Holler, M. Guizar-Sicairos, E. Lima, V. Panneels, G. Pigino, A. G. Bittermann, L. Wettstein, T. Tomizaki, O. Bunk, G. Schertler, T. Ishikawa, R. Wepf, A. Menzel, J. Struct. Biol. 192, 461 (2015), DOI: 10.1016/j.jsb.2015.10.008 First demonstration of ptychographic tomography on frozen hydrated biological cells, this work paved the way to subsequent nanotomography of frozen hydrated biological tissue extending several tens of micrometers in each dimension with quantitative phase contrast. The latter allows the absolute measurement of  the mass density of different cellular compartments, which can be an important analytical tool for biologists. This was demonstrated here using Chlamydomonas cells as a model of a biological sample.