Dr. Valérie Panneels

Scientist
Forschungsstrasse 111
5232 Villigen PSI
Switzerland
Research Interests
Integral membrane proteins like transporters and receptors play key roles in cellular signal transduction. I am interested in better understanding the way receptors orchestrate signaling in complex interactive systems at the molecular level. I am initiating and supervising projects on the structure, function and dynamics of those receptors. Science on eukaryotic membrane proteins requires sophisticated techniques for testing expression, purification, activity and crystallization using amphiphile molecules like detergents and lipids that mimick the membrane. In the group I am therefore involved in experiments using state-of-the-art techniques in biophysics (SLS synchrotron, X-ray free electron laser (SwissFEL), electron microscopy), biochemistry and cell biology for a complete functional and structural characterization of G protein-coupled receptors.
Curriculum Vitae
Publications
2023
- Ultrafast structural changes direct the first molecular events of vision
2022
2021
- Panneels V, Diaz A, Imsand C, Guizar-Sicairos M, Müller E, Bittermann AG, Ishikawa T, Menzel A, Kaech A, Holler M, Grimm C, Schertler G (2021) Imaging of retina cellular and subcellular structures using ptychographic hard X-ray tomography. J. Cell Sci. 2021 Oct 1;134(19):jcs258561. doi: 10.1242/jcs.258561. https://journals.biologists.com/jcs/article-lookup/doi/10.1242/jcs.258561
2020
- Skopintsev P, Ehrenberg D, Weinert T, James D, Kar RK, Johnson PJM, Ozerov D, Furrer A, Martiel I, Dworkowski F, Nass K, Knopp G, Cirelli C, Arrell C, Gashi D, Mous S, Wranik M, Gruhl T, Kekilli D, Brünle S, Deupi X, Schertler GFX, Benoit RM, Panneels V, Nogly P, Schapiro I, Milne C, Heberle J, Standfuss J. (2020) Femtosecond-to-millisecond structural changes in a light-driven sodium pump. Nature 583(7815):314-318. doi: 10.1038/s41586-020-2307-8. Epub 2020 May 20. PMID: 32499654
- Weinert, T., & Panneels, V. (2020). Membrane protein preparation for serial crystallography using high-viscosity injectors: rhodopsin as an example. In C. Perez & T. Maier (Eds.), Methods in molecular biology: Vol. 2127. Expression, purification, and structural biology of membrane proteins (pp. 321-338). https://doi.org/10.1007/978-1-0716-0373-4_21
2019
- Varma, N., Mutt, E., Mühle, J., Panneels, V., Terakita, A., Deupi, X., … Lesca, E. (2019). Crystal structure of jumping spider rhodopsin-1 as a light sensitive GPCR. Proceedings of the National Academy of Sciences of the United States of America PNAS, 116(29), 14574-14556. https://doi.org/10.1073/pnas.1902192116
2018
- Retinal isomerization in bacteriorhodopsin captured by a femtosecond x-ray laser
SCIENCE 361, 145 (2018).DOI: 10.1126/science.aat0094
2017
- Serial millisecond crystallography for routine room-temperature structure determination at synchrotrons
NATURE COMMUNICATIONS 8, 542 (2017).DOI: 10.1038/s41467-017-00630-4
2016
- Addendum to Three-dimensional mass density mapping of cellular ultrastructure by ptychographic X-ray nanotomography [J. Struct. Biol. 192 (2015) 461-469]
JOURNAL OF STRUCTURAL BIOLOGY 193, 83 (2016).DOI: 10.1016/j.jsb.2015.12.003
- Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography
NATURE COMMUNICATIONS 7, 12314 (2016).DOI: 10.1038/ncomms12314
- Three-dimensional mass density mapping of cellular ultrastructure by ptychographic X-ray nanotomography (vol 192, pg 461, 2015)
JOURNAL OF STRUCTURAL BIOLOGY 193, 83-83 (2016).DOI: 10.1016/j.jsb.2015.12.003
2015
- Batch crystallization of rhodopsin for structural dynamics using an X-ray free-electron laser
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS 71, 856 (2015).DOI: 10.1107/S2053230X15009966
- Time-resolved structural studies with serial crystallography: A new light on retinal proteins
STRUCTURAL DYNAMICS 2, 041718 (2015).DOI: 10.1063/1.4922774