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Laboratory for Micro and Nanotechnology

  • About LMN
    • Organisational Structure
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  • Research / Groups
    • X-ray Optics
      • Diffractive X-ray Lenses
        • Electroplated Zone Plates and Beam Shapers
        • Ultra-high Resolution Zone Plates
        • Double-Sided Zone Plates
        • Blazed X-ray Optics
        • Zernike X-ray Phase Contrast Microscopy
      • Grating-based X-ray Interferometry
        • Differential Phase Contrast and Dark-Field Imaging
        • Metrology Applications at Synchrotrons and XFELs
        • Grating Fabrication
      • Diamond X-ray Optics for XFEL Experiments
        • Diamond Zone Plates for XFELs
        • Beam Splitter Gratings for Spectral Monitoring
        • Ultra-Fast Pump-Probe Experiments
    • Polymer Nanotechnology
      • Nanoimprint Lithography
      • Three Dimensional Structures
      • Polymer Grafting
    • SwissFEL nanoengineering
      • Protein Crystallography Using Ultrasonic Acoustic Levitation
      • Supports for Serial Protein Crystallography
        • SwissMX supports
      • Coherent Electron Wavefront Engineering
      • Bio-Interfaces
      • Vacuum Nanoelectronics
        • Research in Field Emitter Arrays
        • Nanotip fabrication
        • Laser induced emission
        • Double gated FEAs
        • Picosecond switching
        • Emittance measurements
        • Uniformity control
    • Molecular Nanoscience
      • On-surface Chemistry
      • Spins in Molecular Monolayers
      • SiC: Surfaces and Interfaces
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      • EUV Interference Lithography
      • EUV Lensless Imaging
      • ALM Nanoscience
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  • Facilities and Equipment
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    • XIL Facility at the SLS
    • Scanning Probe Microscopy
    • Scanning Electron Microscopy
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    • Archive
  • Publications
    • Publications 2011 - 2016

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Dieser Inhalt ist nicht auf Deutsch verfügbar.

Dr. Celestino Padeste

Senior Scientist

Paul Scherrer Institut
Forschungsstrasse 111
5232 Villigen PSI
Schweiz
Telefon
+41 56 310 21 41
E-Mail
celestino.padeste@psi.ch

Open PhD position (method development for time-resolved crystallography) 
The Swiss Nanoscience Institute (SNI) is funding a PhD Project in collaboration with Prof. Thomas R. Ward at University of Basel entitled "Revealing Protein Binding Dynamics Using Time-Resolved Diffraction Experiments at SwissFEL".
Information for applicants for the PhD position is found on the Webpage of the SNI PhD School. 

 

Research

Main interest:
Functional Surfaces and Surface Functionalization

Current Focus:
Combination of advanced micro- and nanostructuring techniques with surface chemistry to obtain surfaces with specific properties.

  • Polymer grafting
    • Creation of nanostructured polymer brushes
    • Adaptation of surface properties of polymer materials
    • Adaptive and switchable surface properties
  • Designed surfaces for bio-applications
    • Micro- and nanopatterns of functional proteins
    • Designed surface topographies for implant research
  • Protein Crystallography
    • Thin-film supports for XFEL-based protein crystallography
    • Nanostructured substrates to induce protein crystallization

Teaching

Chemistry I in the Materials Science Bachelor Degree Course at ETHZ

Publications

Complete List of Publications

​​orcid.org/0000-0001-7577-5781

Recent Papers

  • Górzny MŁ, Opara NL, Guzenko VA, Cadarso VJ, Schift H, Li XD, et al.
    Microfabricated silicon chip as lipid membrane sample holder for serial protein crystallography
    Micro and Nano Engineering. 2019; 3: 31-36. https://doi.org/10.1016/j.mne.2019.03.002
    DORA PSI
  • Makita M, Vartiainen I, Mohacsi I, Caleman C, Diaz A, Jönsson HO, et al.
    Femtosecond phase-transition in hard x-ray excited bismuth
    Scientific Reports. 2019; 9(1): 602 (7 pp.). https://doi.org/10.1038/s41598-018-36216-3
    DORA PSI
  • Moradi M, Opara NL, Tulli LG, Wäckerlin C, Dalgarno SJ, Teat SJ, et al.
    Supramolecular architectures of molecularly thin yet robust free-standing layers
    Science Advances. 2019; 5(2): eaav4489 (7 pp.). https://doi.org/10.1126/sciadv.aav4489
    DORA PSI
  • Casadei CM, Tsai C-J, Barty A, Hunter MS, Zatsepin NA, Padeste C, et al.
    Resolution extension by image summing in serial femtosecond crystallography of two-dimensional membrane-protein crystals
    IUCrJ. 2018; 5(1): 103-117. https://doi.org/10.1107/S2052252517017043
    DORA PSI
  • Opara NL, Mohacsi I, Makita M, Castano-Diez D, Diaz A, Juranić P, et al.
    Demonstration of femtosecond X-ray pump X-ray probe diffraction on protein crystals
    Structural Dynamics. 2018; 5(5): 054303 (15 pp.). https://doi.org/10.1063/1.5050618
    DORA PSI
  • Ranamukhaarachchi SA, Padeste C, Hafeli UO, Stoeber B, Cadarso VJ
    Design considerations of a hollow microneedle-optofluidic biosensing platform incorporating enzyme-linked assays
    Journal of Micromechanics and Microengineering. 2018; 28(2): 24002. https://doi.org/10.1088/1361-6439/aa9c9c
    DORA PSI
  • Seniutinas G, Weber A, Padeste C, Sakellari I, Farsari M, David C
    Beyond 100 nm resolution in 3D laser lithography — post processing solutions
    Microelectronic Engineering. 2018; 191: 25-31. https://doi.org/10.1016/j.mee.2018.01.018
    DORA PSI

Polymer Micro and Nanografting (Book)

micro-nanografting.jpg

Celestino Padeste und Sonja Neuhaus
Polymer Micro- and Nanografting
Based on own work and literature reports this book provides a summary of strategies for the formation of functional polymer patterns and structures on or in polymeric substrates using grafting processes.
Elsevier 2015; ISBN: 978-0-323-35322-9
Online Edition Elsevier Book Store

Selected Papers

Polymer Grafting

M. Dübner, V.J. Cadarso, T.N. Gevrek, A. Sanyal, N.D. Spencer, and C. Padeste
"Reversible Light-Switching of Enzymatic Activity on Orthogonally Functionalized Polymer Brushes"
ACS Appl. Mater. Interfaces 2017, 9 (11), pp 9245-9249.

M. Dübner, M.E. Naoum, N.D. Spencer, C.Padeste,
“From pH- to Light-Response: Post-Polymerization Modification of Polymer Brushes Grafted onto Microporous Polymeric Membranes”,
ACS Omega, 2, 2017, 455-461., ACS LiveSlides™ presentation

K. Gajos, V.A. Guzenko, M. Dübner, J. Haberko, A. Budkowski, C. Padeste
"Electron-beam lithographic grafting of functional polymer structures from fluoropolymer substrates"
Langmuir 32(41), 2016, 10641–10650

G. Panzarasa, M. Dübner, V. Pifferi, G. Soliveri, C. Padeste
"ON/OFF Switching of Silicon Wafer Electrochemistry by pH-Responsive Polymer Brushes."
J. Mater. Chem. C 4,26 (2016) 6287-6294


M. Dübner, T.N. Gevrek, A. Sanyal, N.D. Spencer, C. Padeste,
“Fabrication of Thiol−Ene “Clickable” Copolymer-Brush Nanostructures on Polymeric Substrates via Extreme Ultraviolet Interference Lithography”,
ACS Applied Materials and Interfaces, 7 (2015) 11337−11345

M. Dübner, N.D. Spencer, C. Padeste,
"Light-Responsive Polymer Surfaces via Postpolymerization Modification of Grafted Polymer-Brush Structures",
Langmuir, 30 (2014) 14971−14981

S. Neuhaus, C. Padeste, N.D. Spencer,
“Versatile Wettability Gradients Prepared by Chemical Modification of Polymer Brushes”
Langmuir 27 (2011) 6855-–6861.

S. Neuhaus, C. Padeste, N.D. Spencer,
“Functionalization of Fluropolymers and Polyolefins via Grafting of Polyelectrolyte Brushes from Atmospheric-Pressure Plasma Activated Surfaces”
Plasma Processes and Polymers 8, 6 (2011) 512-522.

S. Neuhaus, C. Padeste, H.H. Solak, N.D. Spencer,
“Functionalization of fluoropolymer surfaces with nanopatterned polyelectrolyte brushes”
Polymer 51 (2010) 4037-4043.

P. Farquet, A. Kunze, C. Padeste, H.H. Solak, S. Alkan Gürsel, G.G. Scherer and A. Wokaun,
“Influence of the solvent viscosity on surface graft-polymerization reactions”,
Polymer, 48 (2007) 4936-4942

H.-P. Brack, C. Padeste, M. Slaski, S. Alkan, and H. H. Solak,
„Preparation of Micro- and Nanopatterns of Polymer Chains Grafted onto Flexible Polymer Substrates”
J. Am. Chem. Soc., 126, 4 (2004) 1004-1005.

Solid supports for serial protein crystallography

M. Ł. Górzny, N.L. Opara, V.A. Guzenko, V.J. Cadarso, H. Schift, X.D. Li, C. Padeste, “Microfabricated silicon chip as lipid membrane sample holder for serial protein crystallography”, Micro and Nano Engineering 3 (2019) 31-36.

N.L. Opara, I. Mohacsi, M. Makita, D. Castano-Diez, A. Diaz, P. Juranić, M. Marsh, A. Meents, C.J. Milne, A. Mozzanica, C. Padeste, V. Panneels, M. Sikorski, S. Song, H. Stahlberg, I. Vartiainen, L. Vera, M. Wang, P.R. Willmott, and C. David “Demonstration of femtosecond X-ray pump X-ray probe diffraction on protein crystals” Structural Dynamics, (2018), 5,5, 054303.

N. Opara, I. Martiel, S.A. Arnold, T. Braun, H. Stahlberg, M. Makita, C. David and C. Padeste
"Direct protein crystallization on ultrathin membranes for diffraction measurements at X-ray free electron lasers."
J. Appl. Cryst. (2017) 50, 909-918. DOI: 10.1107/S1600576717005799

B. Pedrini, C.J. Tsai, G. Capitani, C. Padeste, M.S. Hunter, N.A. Zatsepin, A. Barty, W.H. Benner, S. Boutet, G.K. Feld, S.P. Hau-Riege, R.A. Kirian, C. Kupitz, M. Messerschmitt, J.I. Ogren, T. Pardini, B. Segelke, G.J. Williams, J.C.H. Spence, R. Abela, M. Coleman, J.E. Evans, G. Schertler, M. Frank and X.D. Li,
“7 Å resolution in protein two-dimensional-crystal X-ray diffraction at Linac Coherent Light Source.”
Phil. Trans. R. Soc. B 369 (2014) 20130500.

Bio-functional surfaces and Biosensors

S.A. Ranamukhaarachchi, C. Padeste, M. Dübner, U.O. Häfeli, B. Stoeber, V.J. Cadarso,
Integrated hollow microneedle-optofluidic biosensor for therapeutic drug monitoring in sub-nanoliter volumes
Scientific reports 2016, 6, 29075; DOI: 10.1038/srep29075

J. Althaus, C. Padeste, J. Köser, U. Pieles, K. Peters and B. Müller,
“Nanostructuring Polyetheretherketone for Medical Implants”
European Journal of Nanomedicine 4(1) (2012) 7-15

C. Padeste, H. Özçelik, J. Ziegler, A. Schleunitz, M. Bednarzik, D. Yücel, V. Hasirci,
“Replication of high aspect ratio pillar array structures in biocompatible polymers for tissue engineering applications”,
Microelectron. Engineering, 88 (2011) 1836–1839

C. Padeste, B. Steiger, A. Grubelnik and L. Tiefenauer,
„Molecular Assembly of Redox-Conductive Ferrocene-Streptavidin Conjugates – Towards Bio-Electrochemical Devices”
Biosensors and Bioelectronics 20 (2004) 545-552.

H. Sorribas, C. Padeste and L. Tiefenauer,
"Photolithographic Generation of Protein Micropatterns for Neuron Culture Applications",
Biomaterials 23, 3 (2002) 893-900.

(Nano)-Structuring and Surface Functionalization

S. Neuhaus, N.D. Spencer, C. Padeste,
“Anisotropic Wetting of Microstructured Polymers as a Function of Surface Chemistry”
ACS Applied Materials and Interfaces 4 (2012) 123-127.

A. Savouchkina, A. Foelske-Schmitz, R. Kötz, A. Wokaun, G. G. Scherer, C. Padeste, J. Ziegler, V. Auzelyte, H. H. Solak,
“Extreme Ultraviolet Interference Lithography for Generation of Platinum Nanoparticles on Glassy Carbon”
ECS Transactions, 25, 24 (2010) 175-184.

Short CV

Education
  • Diploma in Chemistry and PhD in inorganic solid state chemistry, University of Zürich, Switzerland
  • Post-Doc in surface science of catalysts, University of New South Wales, Sydney, Australia

Employment At PSI / LMN since August 1993

1993-2003: Molecular Nanotechnology
  • surface design
  • surface modification
  • protein immobilization
  • electrochemical detection of labelled proteins
Since 2003: Nanofabrication Technologies / Polymer Nanotechnology
  • polymer micro- and nanografting
  • x-ray interference lithography (XIL)
  • creation of protein patterns and chemical nanopatterns
responsible for the www-pages of LMN

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Further information

  • PSI Lab for Micro- and Nanotechnology
  • Polymer Grafting
  • Biointerfaces
  • Supports for Protein Crystallography
  • Swiss Light Source
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