Our research aims at developing low-dose electron microscopy techniques to achieve high-resolution 3D imaging while minimizing radiation damage, particularly in biological materials.
We apply cryo-electron microscopy, STEM tomography, 4DSTEM and electron ptychography to study radiation sensitive materials, with a strong emphasis on bio-related applications. In addition, we are exploring methods for volume imaging of thick samples with minimal sample distortion to advance our understanding of complex cellular systems.
One example of our recent work is ptychoScopy, a Python-based software tool that we developed to support the setup and optimization of electron ptychography experiments. The tool helps researchers to navigate key experimental parameters—such as beam convergence, defocus, and dose—to improve reconstruction quality. By guiding users through the complex design process, ptychoScopy facilitates more efficient and successful ptychographic imaging across a range of scientific applications.
References:
Skoupy et al., “Newcomer’s Guide into Optimal Data Acquisition for Electron Ptychography”
Skoupy, R., Müller, E., Pennycook, T.J. et al. Ptychoscopy: a user friendly experimental design tool for ptychography. Sci Rep 15, 24959 (2025). https://doi.org/10.1038/s41598-025-09871-6
Current team members:
Emiliya Poghosyan
Former team members:
Evan Quistad
Radim Skoupy