X06DA - PXIII : Macromolecular Crystallography
X06DA (PXIII) will close on April 11, 2022 for a 1 year upgrade.
Ongoing proposals will be moved to X06SA (PXI).
A Versatile Beamline for Macromolecular Crystallography
X06DA (PXIII) is the third macromolecular crystallography beamline at the Swiss Light Source. It was funded by a partnership between the Paul Scherrer Institut and Swiss and international pharmaceutical companies. The beamline receives light from a 2.9 T superbend magnet. The optical design results in a sub 90 microns x 45 microns focused beam at the sample position with a total photon flux comparable to an undulator beamline. With both the PILATUS 2M-F detector and the multi-axis PRIGo goniometer, the setup is well suited for experimental phasing experiments, in particular native SAD. The experimental station offers both efficient manual mounting with a mini-hutch design and automatic robotic sample mounting.
|Wavelength range (Å)||0.71 - 2.25|
|Spectral range (keV)||5.5 - 17.5|
|Energy resolution||1.4 x 10-4|
|Flux at 12.4 keV at 400mA (ph/s)||5 x 1011|
|Focused spot size h x v (μm)||90 x 45|
|Endstation||Mini-hutch (fast manual mounting), multi-axis PRIGo goniometer,
sample changer CATS
A Phasing Beamline
- Challenging structures solved by native SAD phasing, including both the large 266-kDa multiprotein/multiligand tubulin complex (Weinert et al, 2015, Nature Methods) and a 200-kDa Cas9–RNA–DNA complex (Olieric et al, 2016, ActaD)
- Multi-orientation data collection with the PRIGo goniometer (Waltersperger et al, 2015, JSR)
- Fast high multiplicity with PILATUS 2M-F detector
- Advanced energy interleaved-MAD protocols (Finke et al, 2016, MiMB)
- Rapid access for experimental phasing experiments possible, please contact the beamline staff directly
PILATUS 2M-F detector and the PRIGo goniometer (GIF: Paul Scherrer Institute/Markus Fischer)
A High-Troughput Beamline
- Mini-hutch design enabling fast manual mounting
- 30 second sample exchange time with CATS sample changer from Irelec
- Wet-mounting with caps/vials from Molecular Dimensions