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PXI - X06SA: Macromolecular Crystallography

X06SA (PXI) is the first macromolecular crystallography beamline at the Swiss Light Source. It is equipped with a flexible two-stage focusing X-ray optics system and a single–photon counting hybrid pixel area EIGER 16M (Dectris) detector. X06SA is well suited for micro-crystals, crystallography of large unit cell structures, and single/multiwavelength anomalous diffraction experiments (SAD/MAD).

Beamline Characteristics

Wavelength range (Å)	0.7 - 2.2
Spectral range (keV)	5.7 - 17.5
Energy resolution (%)	0.02
Flux at 12.4 keV at 400mA (ph/s)	> 2 x 10¹²
Spot size h x v (μm)	Fast change from 5 x 5 to 100 x 100, option to focus on detector
	Smallest focusing 10 x 1. Smallest beam 2 x 1 is available upon request
Detector	EIGER 16M X (133 Hz)

Beamline Features

Variable X-ray beam size, divergence, and focusing position

Control of beam size and divergence with a two-stage focusing X-ray optics and a movable secondary source position.

Standard micro-focus 5 x 5 μm² to 100 x 100 μm² (user friendly one button control)
Focus on detector (user friendly one button control)
Micron-size beam, 2 x 1 μm² (upon request)

EIGER 16M: the most powerful X-ray detector for synchrotron application

75 x 75 μm² pixel size, 3.8 μs dead time, 133 Hz frame rate, maximum count rate 5x10⁸ phs/s/mm²

High sensibility with single photon counting and zero point-spread
High spacial resolution to high diffraction resolution with smaller pixel
Ultrafine-phi slicing with > 99% duty cycle, i.e. < 1% "non-recorded" photons

Continuous grid scan (up to 60 Hz)

Diffraction-based alignment
Fast grid-scan with micro-beam (micro-rastering)
Wojdyla et al., J. Appl. Cryst. 49, 944 (2016) DOI:10.1107/S1600576716006233

IMISX serial crystallography for membrane and soluble protein

Eliminate the technically demanding and inefficient crystal-harvesting process in meso
Harvest, freeze, store and transport IMISX wells in standard cryo pin, vial, puck and dewar
Visualize micro-crystals in meso in cryo
Huang et al. Acta Cryst. D71, 1238 (2015) DOI:10.1107/S1399004715005210; Acta Cryst. D72, 93 (2016) 10.1107/S2059798315021683

Serial synchrotron crystallography (SSX) software suite at PXI and PXII

identification of well diffracting crystals with fast grid scan in DA+ GUI
evaluation of grid scan results and automated collection of multiple minisets performed in the CY+ GUI
automatic data processing (adp) of individual minisets
automatic data merging (adm) of multiple minisets using the sxdm package
automatic display of adp and adm results for the on-the- fly inspection (adp-tracker) and storage in the database (mxdb)
Basu et al., J. Synchrotron Radiat., 2019, 26(1):244-252 DOI:http://doi.org/10.1107/S1600577518016570

TELL sample changer

20 seconds sample exchange time
dry mounting, standard 18 mm SPINE pins
Unipucks, NO SPINE pucks at 6S for use with TELL!!!
dewar capacity: 30 Unipucks

SLS PX software

easy and intuitive data acquisition with DA+ GUI
robust DA+ data acquisition engine (DA+ server and Escape)
SLS MX MonogDB database (so-called mxdb)
automated data processing (adp) pipelines for single data set, multi-orientation data sets and serial crystallography data
automated data merging (adm) for serial crystallography data with in-house sxdm pipeline
Wojdyla et al., J. Synchr. Rad. 25, 293 (2018) DOI:10.1107/S1600577517014503