PSI light microscopy facility: Instrumentation

• Obj. HC PL APO 20x/0.75 CS2
• Obj. HC PL APO 63x/1.40 OIL CS2
• Laser: 405/ 488/ 561/ 638 nm

Acquisition year: 2020

Confocal microscope
Widefield option

Objectives:

• CFI Plan Apochromat Lambda 10X N.A. 0.45, W.D. 4.0 mm
• CFI Plan Apochromat Lambda 20X N.A. 0.75, W.D. 1.0 mm
• CFI Plan Apochromat Lambda 40X N.A. 0.95, W.D. 0.21 mm
• CFI Plan Apochromat Lambda 40XC Silicon, N.A. 1.25, W.D. 0.30 mm
• CFI Plan Apochromat Lambda 60xH NA1.40, WD 0.13mm
• CFI Plan Apochromat 100X Lambda Oil, NA 1.45

Laser: 405/ 488/ 561/ 640 nm

Acquisition year: 2020

Widefield microscope
TIRF with 4 lasers

Objectives:

• LUCPLFLN PH 20x / 0.45
• Zeiss 40x
• PLAPO O3PH 60x / 1.4 Oil
• UPLAPO OI3PH 100x / 1.35 / Oil
• UAPON OTIRF 100x / 1.49 / Oil
•  

Laser: 405/ 488/ 561/ 640 nm

Upgrade: 2020

• Objectives: 10x, 20x, low resolution
• Channels: DAPI/ FITC/ TRITC/ Cy5

Acquisition year: 2021

Objectives:

• CFI Plan Apochromat LambdaD 10X
• CFI Plan Apochromat Lambda D 20X *
• CFI Plan Apochromat Lambda S25xC Sil N.A. 1.05, W.D. 0.55 mm
• CFI Plan Fluor 40X Oil N.A. 1.30, W.D. 0.2mm *
• CFI Plan Apochromat Lambda D 60X Oil *
     * DeepSIM calibrated

Laser: 405/ 446/ 476/ 518/ 545/ 637/ 748 nm

Acquisition year: 2024

Objectives:

• CFI Plan Apochromat Lambda D 4X
• CFI Plan Apochromat Lambda D 10X
• CFI Plan Apochromat Lambda D 20X
• CFI Plan Apochromat Lambda D 40X

Laser: 405/ 488/ 561/ 640 nm

Acquisition year: 2024

Acquisition year: 2024

Objectives:

• CFI Plan Apochromat Lambda D 4X
• CFI Plan Apochromat Lambda D 10X
• CFI Plan Apochromat Lambda D 20X
• CFI Plan Apochromat Lambda D 40X
• CFI Plan Apochromat Lambda D 60X Oil
• CFI Plan Apochromat Lambda D 100x Oil

Wide field fluorescence microscope
DAPI/ FITC/ TRITC/ Cherry/ Cy5
Fully motorized

Iterative staining of tissue sections with up to 100 antibodies
Includes micro-fluidics and automated image acquisition
Wide field fluorescence microscope

Objectives:

• 10X
• 20X
• 40X

Acquisition year: 2024

Flow cytometer, no sorting

4 lasers, up to 14 channels

Brightfield camera

Acquisition year: 2024