Protocols
Crystallizing membrane proteins using lipidic mesophases
Caffrey M, Cherezov V
Nature Protocols 4, 706–731 (2009).
DOI: 10.1038/nprot.2009.31
Crystallization of Membrane Proteins in Lipidic Mesophases
Liu W, Cherezov V
J. Vis. Exp. 49 (2011).
DOI: 10.3791/2501
Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography
Ishchenko A, Cherezov V, Liu W
J. Vis. Exp. 115 (2016).
DOI: 10.3791/54463
Selected Publications
Retinal isomerization in bacteriorhodopsin captured by a femtosecond x-ray laser
Nogly P, Weinert T, James D, Carbajo S, Ozerov D, Furrer A, Gashi D, Borin V, Skopintsev P, Jaeger K, Nass K, Båth P, Bosman R, Koglin J, Seaberg M, Lane T, Kekilli D, Brünle S, Tanaka T, Wu W, Milne C, White T, Barty A, Weierstall U, Panneels V, Nango E, Iwata S, Hunter M, Schapiro I, Schertler G, Neutze R, Standfuss J
SCIENCE 361, 145 (2018).
DOI: 10.1126/science.aat0094
Serial millisecond crystallography for routine room-temperature structure determination at synchrotrons
Weinert T, Olieric N, Cheng R, Brünle S, James D, Ozerov D, Gashi D, Vera L, Marsh M, Jaeger K, Dworkowski F, Panepucci E, Basu S, Skopintsev P, Doré Andrew S, Geng T, Cooke Robert M, Liang M, Prota Andrea E, Panneels V, Nogly P, Ermler U, Schertler G, Hennig M, Steinmetz Michel O, Wang M, Standfuss J
NATURE COMMUNICATIONS 8, 542 (2017).
DOI: 10.1038/s41467-017-00630-4
Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography
Nogly P, Panneels V, Nelson G, Gati C, Kimura T, Milne C, Milathianaki D, Kubo M, Wu W, Conrad C, Coe J, Bean R, Zhao Y, Båth P, Dods R, Harimoorthy R, Beyerlein Kenneth R, Rheinberger J, James D, DePonte D, Li C, Sala L, Williams Garth J, Hunter Mark S, Koglin Jason E, Berntsen P, Nango E, Iwata S, Chapman Henry N, Fromme P, Frank M, Abela R, Boutet S, Barty A, White Thomas A, Weierstall U, Spence J, Neutze R, Schertler G, Standfuss J
NATURE COMMUNICATIONS 7, 12314 (2016).
DOI: 10.1038/ncomms12314
A three-dimensional movie of structural changes in bacteriorhodopsin
Nango E, Royant A, Kubo M, Nakane T, Wickstrand C, Kimura T, Tanaka T, Tono K, Song C, Tanaka R, Arima T, Yamashita A, Kobayashi J, Hosaka T, Mizohata E, Nogly P, Sugahara M, Nam D, Nomura T, Shimamura T, Im D, Fujiwara T, Yamanaka Y, Jeon B, Nishizawa T, Oda K, Fukuda M, Andersson R, Båth P, Dods R, Davidsson J, Matsuoka S, Kawatake S, Murata M, Nureki O, Owada S, Kameshima T, Hatsui T, Joti Y, Schertler G, Yabashi M, Bondar A, Standfuss J, Neutze R, Iwata S
SCIENCE 354 (2016).
DOI: 10.1126/science.aah3497
Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography
Weierstall U, James D, Wang C, White TA, Wang D, Liu W, Spence JC, Bruce Doak R, Nelson G, Fromme P, Fromme R, Grotjohann I, Kupitz C, Zatsepin NA, Liu H, Basu S, Wacker D, Han GW, Katritch V, Boutet S, Messerschmidt M, Williams GJ, Koglin JE, Marvin Seibert M, Klinker M, Gati C, Shoeman RL, Barty A, Chapman HN, Kirian RA, Beyerlein KR, Stevens RC, Li D, Shah ST, Howe N, Caffrey M, Cherezov V.
NATURE COMMUNICATIONS 5, 3309 (2014).
DOI: 10.1038/ncomms4309
2019
- Arrestin-1 engineering facilitates complex stabilization with native rhodopsin
Scientific Reports 9, 439 (2019).DOI: 10.1038/s41598-018-36881-4
2018
- CLASP Suppresses Microtubule Catastrophes through a Single TOG Domain.
DEVELOPMENTAL CELL 46, 40 (2018).
- Crystal structure of rhodopsin in complex with a mini-G o sheds light on the principles of G protein selectivity
Science Advances 4, eaat7052 (2018).DOI: 10.1126/sciadv.aat7052
- In situ serial crystallography for rapid de novo membrane protein structure determination.
Communications Biology 1, 124 (2018).DOI: 10.1038/S42003-018-0123-6
- Ligand channel in pharmacologically stabilized rhodopsin.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 115, 3640 (2018).
- Retinal isomerization in bacteriorhodopsin captured by a femtosecond x-ray laser
SCIENCE 361, 145 (2018).DOI: 10.1126/science.aat0094
2017
- Perspective: Opportunities for ultrafast science at SwissFEL
STRUCTURAL DYNAMICS 4, 061602 (2017).DOI: 10.1063/1.4997222
- Role of the nucleotidyl cyclase helical domain in catalytically active dimer formation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 114, E9821 (2017).DOI: 10.1073/PNAS.1712621114
- Serial crystallography at synchrotrons and X-ray lasers
IUCRJ 4, 100 (2017).
- Serial millisecond crystallography for routine room-temperature structure determination at synchrotrons
NATURE COMMUNICATIONS 8, 542 (2017).DOI: 10.1038/s41467-017-00630-4
- Structural biology Signalling under the microscope
NATURE 546, 36-37 (2017).DOI: 10.1038/nature22491
- Structure of the Full-length VEGFR-1 Extracellular Domain in Complex with VEGF-A.
STRUCTURE 25, 341 (2017).DOI: 10.1016/J.STR.2016.12.012
2016
- A three-dimensional movie of structural changes in bacteriorhodopsin
SCIENCE 354, 1552-1557 (2016).DOI: 10.1126/science.aah3497
- Data-collection strategy for challenging native SAD phasing
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY 72, 421 (2016).DOI: 10.1107/S2059798315024110
- Functional map of arrestin binding to phosphorylated opsin, with and without agonist
Scientific Reports 6, 28686 (2016).DOI: 10.1038/srep28686
- Functional map of arrestin binding to phosphorylated opsin, with and without agonist (vol 6, 28686, 2016)
Scientific Reports 6, 30224 (2016).DOI: 10.1038/srep30224
- In meso in situ In meso in situ serial X-ray crystallography of soluble and membrane proteins at cryogenic temperatures
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY 72, 93-112 (2016).DOI: 10.1107/S2059798315021683
- In meso in situ serial X-ray crystallography of soluble and membrane proteins at cryogenic temperatures.
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY 72, 93 (2016).DOI: 10.1107/S2059798315021683
- Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography
NATURE COMMUNICATIONS 7, 12314 (2016).DOI: 10.1038/ncomms12314
- Serial Millisecond Crystallography of Membrane Proteins
ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY 922, 137 (2016).DOI: 10.1007/978-3-319-35072-1_10
- Structural role of the T94I rhodopsin mutation in congenital stationary night blindness
EMBO REPORTS 17, 1431-1440 (2016).DOI: 10.15252/embr.201642671