The PSI Protein Production Platform (P4)

The PSI Protein Production Platform (P4) is a research facility specialized in recombinant protein production, with state-of-the-art equipment and a team of highly skilled and dedicated professionals with extensive experience in protein expression and purification. We offer recombinant protein production in bacterial, yeast, protozoan, insect and mammalian cells.

The principal aim of P4 is to support research groups at LBR and to produce high-quality protein for high-resolution structure determination by X-ray crystallography with a particular focus on membrane proteins and cytoskeletal proteins. Furthermore, P4 collaborates with research groups in Switzerland and world-wide and also offers recombinant protein production on a fee-for-service basis.

Together with the PSI Crystallization Facility, P4 represents the “gene-to-structure” pipeline designed to cover all steps from cloning to rapid mutant generation, biophysical and functional studies, as well as X-ray data collection at the SLS and high-resolution structure determination. Access to facilities for European users with an interest in membrane protein expression and/or crystallization is funded by the BioStruct-X programme.

We offer:

Project Design

P4 staff have extensive experience in recombinant protein production. We aim to provide the best possible advice on construct design, expression systems and vectors for each project to obtain optimal results.

Molecular Cloning:

A range of molecular services including construct design, cloning gene of interest into expression vector, site-directed mutagenesis, specialised expression vectors and vector development are available. More…

Protein Expression:

A range of host systems such as bacterial, yeast (Pichia pastoris), protozoan, insect cell/baculovirus and mammalian cell expression (HEK293) are used to achieve optimal expression results. More…

Protein Purification:

A range of techniques such as affinity, size exclusion and ion exchange chromatography are used to obtain optimal protein purification results. Results are routinely analysed by Western blotting and mass spectrometry. More…

Protein Characterization:

A range of biophysical techniques such as multi angle light scattering, analytical ultracentrifugation (instrument equipped with fluorescence option), circular dichroism and isothermal titration calorimetry are available for state-of-the-art protein characterization. More…