Research by Guido Capitaniwww.eppic-web.org), linked to a database (EPPIC db) that contains precomputed analysis results (Baskaran et al., 2014) for nearly one million interfaces as of April 2016.
Experimentally, we are involved, in collaboration with the Glockshuber group (ETHZ), in structural studies of a proteinaceous organelle, the type 1 pilus from uropathogenic Escherichia coli (UPEC), and in particular of its main subunit FimA. The type 1 pilus enables UPEC to infect humans by attaching to sugar moieties that reside on the urethral epithelium. It represents a very interesting system to study hybrid approaches to structure determination and structure-function relationships.
Representative publicationsUnderstanding the fabric of protein crystals: computational classification of biological interfaces and crystal contacts.
Capitani G, Duarte JM, Baskaran K, Bliven S and Somody J
Bioinformatics (Oxford) 2016 32:481-9.
"Protein interface classification by evolutionary analysis."
Duarte JM, Srebniak A, Schärer MA and Capitani G
BMC Bioinformatics 2012 13:334.
EPPIC (Evolutionary Protein-Protein Interface Classifier) web server: www.eppic-web.org
"Structural basis of chaperone-subunit complex recognition by the type 1 pilus assembly platform FimD."
Nishiyama M, Horst R, Eidam O, Herrmann T, Ignatov O, Vetsch M, Bettendorff P, Jelesarov I, Grütter MG, Wüthrich K, Glockshuber R and Capitani G
EMBO J 2005 24:2075-86.