Biophysical InstrumentsBMR has extensive expertise in biophysical techniques used to characterise soluble as well as membrane proteins. Main questions concern protein stability, the size of proteins and their oligomeric state. We also study how the protein stability or the interactions within protein complexes are affected by sample conditions with the goal to optimise conditions for structural studies.
The facility is supervised by Dr. Dmitry Veprintsev.
Nanodrop ND-1000 is used measure protein and DNA concentration in small volume (1-2 uL), significantly reducing sample requirements. It is especially suited for concentrated samples.
Cary 50 allows accurate measurements of more dilute samples.
Cary 300 is equipped with thermostated multi-position cuvette holder and incorporated magnetic stirrers. This allows monitoring stability of several protein samples or enzymatic reactions in parallel.
Varian Eclipse is a modern ultrasensitive benchtop fluorimeter. It is equipped with motorised polarisers to record fluorescence anisotropy, a multi-position thermostated magnetically stirred cuvette holder and automated titrators to perform binding titration experiments. It is also equipped with thermocouples to monitor the temperature in the cuvettes during temperature ramping experiments.
Fluorescence Nanodrop ND-3300 allows recording fluorescence spectra from small volume samples (1-2 uL).
Plate reader Tecan UltraEvolution - this plate reader combines absorbance and several fluorescence detection modes such as fluorescence intensity, fluorescence anisotropy and time-resolved fluorescence with ability to read 96 and 384 well plates.