Dr. Natacha Gaillard
Paul Scherrer Institute
5232 Villigen PSI
5232 Villigen PSI
Microtubules are dynamic filaments composed of -tubulin and implicated in a wide range of cellular processes. They are involved in the organization of intracellular structures and in the intracellular transport, drive chromosome segregation during cell division, influence cell polarity and morphogenesis, and form the core of cilia and flagella. Centrioles are evolutionary conserved, barrel-shaped structures made up of nine sets of microtubule triplets and are fundamental for the assembly of cilia and flagella. Structural or functional centriole aberrations are associated with ciliopathies, a variety of complex diseases that stem from the absence or the dysfunction of cilia. Understanding centriole assembly and architecture is a prerequisite in order to better understand human diseases related to centriole aberrations. I use structural biology techniques (X-ray crystallography, serial crystallography at the synchrotron, electron microscopy) in combination with biophysical, biochemical and cell biology approaches to elucidate the molecular mechanisms involved in centriole/basal body biogenesis and structure.
CLASP Suppresses Microtubule Catastrophes through a Single TOG Domain.
DEVELOPMENTAL CELL 46, 40 (2018).
Cep120 promotes microtubule formation through a unique tubulin binding C2 domain
JOURNAL OF STRUCTURAL BIOLOGY -, S1047847718300248 (2018).DOI: 10.1016/j.jsb.2018.01.009
Structure-activity relationships, biological evaluation and structural studies of novel pyrrolonaphthoxazepines as antitumor agents.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY 162, 290 (2018).
Sustainable Syntheses of (-)-Jerantinines A & E and Structural Characterisation of the Jerantinine-Tubulin Complex at the Colchicine Binding Site
Scientific Reports 8, 10617 (2018).DOI: 10.1038/s41598-018-28880-2
Identification of Chlamydomonas Central Core Centriolar Proteins Reveals a Role for Human WDR90 in Ciliogenesis
CURRENT BIOLOGY 27, 2486 (2017).DOI: 10.1016/j.cub.2017.07.011
Quinolin-6-Yloxyacetamides Are Microtubule Destabilizing Agents That Bind to the Colchicine Site of Tubulin
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 18, 1336 (2017).DOI: 10.3390/ijms18071336
Self-assembled alpha-Tocopherol Transfer Protein Nanoparticles Promote Vitamin E Delivery Across an Endothelial Barrier.
Scientific Reports 7, 4970 (2017).DOI: 10.1038/S41598-017-05148-9
Serial millisecond crystallography for routine room-temperature structure determination at synchrotrons
NATURE COMMUNICATIONS 8, 542 (2017).DOI: 10.1038/s41467-017-00630-4
Triazolopyrimidines Are Microtubule-Stabilizing Agents that Bind the Vinca Inhibitor Site of Tubulin.
CELL CHEMICAL BIOLOGY 24, 737 (2017).DOI: 10.1016/J.CHEMBIOL.2017.05.016
Data-collection strategy for challenging native SAD phasing
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY 72, 421 (2016).DOI: 10.1107/S2059798315024110
Kinesin-Binding Protein Controls Microtubule Dynamics and Cargo Trafficking by Regulating Kinesin Motor Activity
CURRENT BIOLOGY 26, 849 (2016).DOI: 10.1016/j.cub.2016.01.048
Structural basis for misregulation of kinesin KIF21A autoinhibition by CFEOM1 disease mutations
Scientific Reports 6, 30668 (2016).DOI: 10.1038/srep30668
The Human Centriolar Protein CEP135 Contains a Two-Stranded Coiled-Coil Domain Critical for Microtubule Binding
STRUCTURE 24, 1358 (2016).DOI: 10.1016/j.str.2016.06.011
The synthetic diazonamide DZ-2384 has distinct effects on microtubule curvature and dynamics without neurotoxicity
SCIENCE TRANSLATIONAL MEDICINE 8, 365ra159 (2016).DOI: 10.1126/scitranslmed.aag1093
Native Architecture of the Centriole Proximal Region Reveals Features Underlying Its 9-Fold Radial Symmetry
CURRENT BIOLOGY 23, 1620 (2013).DOI: 10.1016/j.cub.2013.06.061
Structural Basis of the 9-Fold Symmetry of Centrioles
CELL 144, 364 (2011).DOI: 10.1016/j.cell.2011.01.008
Automated seamless DNA co-transformation cloning with direct expression vectors applying positive or negative insert selection
BMC BIOTECHNOLOGY 10, 56 (2010).DOI: 10.1186/1472-6750-10-56
Automated unrestricted multigene recombineering for multiprotein complex production
NATURE METHODS 6, 447 (2009).DOI: 10.1038/nmeth.1326