Dr. Natacha Gaillard

Scientist

Paul Scherrer Institute
Forschungsstrasse 111
5232 Villigen PSI
Suisse

Microtubules are dynamic filaments composed of -tubulin and implicated in a wide range of cellular processes. They are involved in the organization of intracellular structures and in the intracellular transport, drive chromosome segregation during cell division, influence cell polarity and morphogenesis, and form the core of cilia and flagella. Centrioles are evolutionary conserved, barrel-shaped structures made up of nine sets of microtubule triplets and are fundamental for the assembly of cilia and flagella. Structural or functional centriole aberrations are associated with ciliopathies, a variety of complex diseases that stem from the absence or the dysfunction of cilia. Understanding centriole assembly and architecture is a prerequisite in order to better understand human diseases related to centriole aberrations.  I use structural biology techniques (X-ray crystallography, serial crystallography at the synchrotron, electron microscopy) in combination with biophysical, biochemical and cell biology approaches to elucidate the molecular mechanisms involved in centriole/basal body biogenesis and structure. 

2018

  • CLASP Suppresses Microtubule Catastrophes through a Single TOG Domain. Aher A, Kok M, Sharma A, Rai A, Olieric N, Rodriguez-Garcia R, Katrukha EA, Weinert T, Olieric V, Kapitein LC, Steinmetz MO, Dogterom M, Akhmanova A
    DEVELOPMENTAL CELL 46, 40 (2018).
  • Cep120 promotes microtubule formation through a unique tubulin binding C2 domain Sharma A, Gerard Samuel F, Olieric N, Steinmetz Michel O
    JOURNAL OF STRUCTURAL BIOLOGY -, S1047847718300248 (2018).
    DOI: 10.1016/j.jsb.2018.01.009
  • Structure-activity relationships, biological evaluation and structural studies of novel pyrrolonaphthoxazepines as antitumor agents. Brindisi M, Ulivieri C, Alfano G, Gemma S, de Asis Balaguer F, Khan T, Grillo A, Chemi G, Menchon G, Prota AE, Olieric N, Lucena-Agell D, Barasoain I, Diaz JF, Nebbioso A, Conte M, Lopresti L, Magnano S, Amet R, Kinsella P, Zisterer DM, Ibrahim O, OSullivan J, Morbidelli L, Spaccapelo R, Baldari C, Butini S, Novellino E, Campiani G, Altucci L, Steinmetz MO, Brogi S
    EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY 162, 290 (2018).
  • Sustainable Syntheses of (-)-Jerantinines A & E and Structural Characterisation of the Jerantinine-Tubulin Complex at the Colchicine Binding Site Smedley Christopher J, Stanley Paul A, Qazzaz Mohannad E, Prota Andrea E, Olieric N, Collins H, Eastman H, Barrow Andrew S, Lim K, Kam T, Smith Brian J, Duivenvoorden Hendrika M, Parker Belinda S, Bradshaw Tracey D, Steinmetz Michel O, Moses John E
    Scientific Reports 8, 10617 (2018).
    DOI: 10.1038/s41598-018-28880-2

2017

  • Identification of Chlamydomonas Central Core Centriolar Proteins Reveals a Role for Human WDR90 in Ciliogenesis Hamel V, Steib E, Hamelin R, Armand F, Borgers S, Flückiger I, Busso C, Olieric N, Sorzano Carlos Oscar S, Steinmetz Michel O, Guichard P, Gönczy P
    CURRENT BIOLOGY 27, 2486 (2017).
    DOI: 10.1016/j.cub.2017.07.011
  • Quinolin-6-Yloxyacetamides Are Microtubule Destabilizing Agents That Bind to the Colchicine Site of Tubulin Sharma A, Sáez-Calvo G, Olieric N, de Asís Balaguer F, Barasoain I, Lamberth C, Díaz J, Steinmetz M
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 18, 1336 (2017).
    DOI: 10.3390/ijms18071336
  • Self-assembled alpha-Tocopherol Transfer Protein Nanoparticles Promote Vitamin E Delivery Across an Endothelial Barrier. Aeschimann W, Staats S, Kammer S, Olieric N, Jeckelmann JM, Fotiadis D, Netscher T, Rimbach G, Cascella M, Stocker A
    Scientific Reports 7, 4970 (2017).
    DOI: 10.1038/S41598-017-05148-9
  • Serial millisecond crystallography for routine room-temperature structure determination at synchrotrons Weinert T, Olieric N, Cheng R, Brünle S, James D, Ozerov D, Gashi D, Vera L, Marsh M, Jaeger K, Dworkowski F, Panepucci E, Basu S, Skopintsev P, Doré Andrew S, Geng T, Cooke Robert M, Liang M, Prota Andrea E, Panneels V, Nogly P, Ermler U, Schertler G, Hennig M, Steinmetz Michel O, Wang M, Standfuss J
    NATURE COMMUNICATIONS 8, 542 (2017).
    DOI: 10.1038/s41467-017-00630-4
  • Triazolopyrimidines Are Microtubule-Stabilizing Agents that Bind the Vinca Inhibitor Site of Tubulin. Saez-Calvo G, Sharma A, Balaguer FA, Barasoain I, Rodriguez-Salarichs J, Olieric N, Munoz-Hernandez H, Berbis MA, Wendeborn S, Penalva MA, Matesanz R, Canales A, Prota AE, Jimenez-Barbero J, Andreu JM, Lamberth C, Steinmetz MO, Diaz JF
    CELL CHEMICAL BIOLOGY 24, 737 (2017).
    DOI: 10.1016/J.CHEMBIOL.2017.05.016

2016

  • Data-collection strategy for challenging native SAD phasing Olieric V, Weinert T, Finke Aaron D, Anders C, Li D, Olieric N, Borca Camelia N, Steinmetz Michel O, Caffrey M, Jinek M, Wang M
    ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY 72, 421 (2016).
    DOI: 10.1107/S2059798315024110
  • Kinesin-Binding Protein Controls Microtubule Dynamics and Cargo Trafficking by Regulating Kinesin Motor Activity Kevenaar Josta T, Bianchi S, van Spronsen M, Olieric N, Lipka J, Frias Cátia P, Mikhaylova M, Harterink M, Keijzer N, Wulf Phebe S, Hilbert M, Kapitein Lukas C, de Graaff E, Ahkmanova A, Steinmetz Michel O, Hoogenraad Casper C
    CURRENT BIOLOGY 26, 849 (2016).
    DOI: 10.1016/j.cub.2016.01.048
  • Structural basis for misregulation of kinesin KIF21A autoinhibition by CFEOM1 disease mutations Bianchi S, van Riel Wilhelmina E, Kraatz Sebastian HW, Olieric N, Frey D, Katrukha Eugene A, Jaussi R, Missimer J, Grigoriev I, Olieric V, Benoit Roger M, Steinmetz Michel O, Akhmanova A, Kammerer Richard A
    Scientific Reports 6, 30668 (2016).
    DOI: 10.1038/srep30668
  • The Human Centriolar Protein CEP135 Contains a Two-Stranded Coiled-Coil Domain Critical for Microtubule Binding Kraatz S, Guichard P, Obbineni Jagan M, Olieric N, Hatzopoulos Georgios N, Hilbert M, Sen I, Missimer J, Gönczy P, Steinmetz Michel O
    STRUCTURE 24, 1358 (2016).
    DOI: 10.1016/j.str.2016.06.011
  • The synthetic diazonamide DZ-2384 has distinct effects on microtubule curvature and dynamics without neurotoxicity Wieczorek Michal, Tcherkezian Joseph, Bernier Cynthia, Prota AE, Chaaban Sami, Rolland Yanneve, Godbout Claude, Hancock Mark A, Arezzo Joseph C, Ocal Ozhan, Rocha Cecilia, Olieric N, Hall Anita, Ding Hui, Bramoulle Alexandre, Annis Matthew G, Zogopoulos George, Harran Patrick G, Wilkie Thomas M, Brekken Rolf A, Siegel Peter M, Steinmetz MO, Shore Gordon C, Brouhard Gary J, Roulston Anne
    SCIENCE TRANSLATIONAL MEDICINE 8, 365ra159 (2016).
    DOI: 10.1126/scitranslmed.aag1093

2013

  • Native Architecture of the Centriole Proximal Region Reveals Features Underlying Its 9-Fold Radial Symmetry Guichard P, Hachet V, Majubu N, Neves A, Demurtas D, Olieric N, Fluckiger I, Yamada A, Kihara K, Nishida Y, Moriya S, Steinmetz Michel O, Hongoh Y, Gönczy P
    CURRENT BIOLOGY 23, 1620 (2013).
    DOI: 10.1016/j.cub.2013.06.061

2011

  • Structural Basis of the 9-Fold Symmetry of Centrioles Kitagawa D, Vakonakis I, Olieric N, Hilbert M, Keller D, Olieric V, Bortfeld M, Erat MC, Fluckiger I, Gonczy P, Steinmetz MO
    CELL 144, 364 (2011).
    DOI: 10.1016/j.cell.2011.01.008

2010

  • Automated seamless DNA co-transformation cloning with direct expression vectors applying positive or negative insert selection Olieric N, Kuchen M, Wagen S, Sauter M, Crone S, Edmondson S, Frey D, Ostermeier C, Steinmetz M, Jaussi R
    BMC BIOTECHNOLOGY 10, 56 (2010).
    DOI: 10.1186/1472-6750-10-56

2009

  • Automated unrestricted multigene recombineering for multiprotein complex production Bieniossek C, Nie Y, Frey D, Olieric N, Schaffitzel C, Collinson I, Romier C, Berger P, Richmond T, Steinmetz M, Berger I
    NATURE METHODS 6, 447 (2009).
    DOI: 10.1038/nmeth.1326