Dr. Natacha Gaillard


Paul Scherrer Institute
Forschungsstrasse 111
5232 Villigen PSI

Natacha Gaillard completed her PhD at the University of Strasbourg in Structural Biology under the supervision of Prof. Jean Cavarelli (IGBMC, Institut de Génétique et de Biologie Moléculaire et Cellulaire, Strasbourg, France). In 2007 she joined the PSI (Paul Scherrer Institute, Villigen, Switzerland) on the Molecular Biology High Throughput Platform as a post-doc working on kinetochore proteins. In 2011 she became Scientist in the group of Prof. Michel Steinmetz.

In 2017, with her colleague Ashwani Sharma, they initiated a new topic on infectious diseases.

Natacha chose the microtubule cytoskeleton as field of research. She has applied her technical skills to collaborate with several teams internally to develop several technologies for which the PSI is at the forefront of research (ex. sulfur-SAD phasing - SwissFEL for Biological samples - sample solid support for X-ray technologies) and her scientific approaches on various topics like centrosome biology, kinesin regulation and anti-cancer microtubule targeting agents.

Initiated in 2017 with the support of Novartis, Natacha Gaillard in collaboration with her colleague Ashwani Sharma, have started a new topic on infectious diseases aiming at understanding the parasite-host interaction tackling two main lines of research: study and understand the Biology of Apicomplexan parasites and identify new putative targets for drug development.

Expert for the Swiss Youth Research Foundation

Biology and Medicine Section.

Grant SNSF Spark (Rapid Funding of unconventional Ideas)

Host cytoskeleton manipulation by Apicomplexan parasites: structural and functional investigation of the RON Complex.

Mentor for the ETH Program Fix the Leaky Pipeline

Career building program for women in Science

Grant Novartis FreeNovation (funding program for unconventional and creative projects)

Towards the structure based design of broad spectrum anti-apicomplexan drugs.

Natacha Gaillard since 2022 - Previously Natacha Olieric

  • Mühlethaler T, Olieric N, Ehrhard VA, Wranik M, Standfuss J, Sharma A, et al.
    Crystallization systems for the high-resolution structural analysis of tubulin-ligand complexes
    In: Inaba H, ed. Microtubules. Methods and protocols. Methods in molecular biology. New York: Humana Press; 2022:349-374. https://doi.org/10.1007/978-1-0716-1983-4_23
  • Gaillard N, Sharma A, Abbaali I, Liu T, Shilliday F, Cook AD, et al.
    Inhibiting parasite proliferation using a rationally designed anti-tubulin agent
    EMBO Molecular Medicine. 2021; 13(11): e13818 (12 pp.). https://doi.org/10.15252/emmm.202013818
  • Mühlethaler T, Olieric N, Ehrhard VA, Wranik M, Standfuss J, Sharma A, et al.
    Crystallization systems for the high-resolution structural analysis of tubulin-ligand complexes
    In: Inaba H, ed. Microtubules. Methods and protocols. Methods in molecular biology. New York: Humana Press; 2022:349-374. https://doi.org/10.1007/978-1-0716-1983-4_23
  • Gao L, Meiring JCM, Kraus Y, Wranik M, Weinert T, Pritzl SD, et al.
    A robust, GFP-orthogonal photoswitchable inhibitor scaffold extends optical control over the microtubule cytoskeleton
    Cell Chemical Biology. 2021; 28(2): 228-241. https://doi.org/10.1016/j.chembiol.2020.11.007
  • Martiel I, Beale JH, Karpik A, Huang C-Y, Vera L, Olieric N, et al.
    Versatile microporous polymer-based supports for serial macromolecular crystallography
    Acta Crystallographica Section D: Structural Biology. 2021; 77(9): 1153-1167. https://doi.org/10.1107/S2059798321007324
  • Sharma A, Olieric N, Steinmetz MO
    Centriole length control
    Current Opinion in Structural Biology. 2021; 66: 89-95. https://doi.org/10.1016/j.sbi.2020.10.011
  • Atherton J, Hummel JJA, Olieric N, Locke J, Peña A, Rosenfeld SS, et al.
    The mechanism of kinesin inhibition by kinesin binding protein
    eLife. 2020; 9: e61481 (30 pp.). https://doi.org/10.7554/eLife.61481
  • Nass K, Cheng R, Vera L, Mozzanica A, Redford S, Ozerov D, et al.
    Advances in long-wavelength native phasing at X-ray free-electron lasers
    IUCrJ. 2020; 7: 965-975. https://doi.org/10.1107/S2052252520011379
  • Rodríguez-García R, Volkov VA, Chen C-Y, Katrukha EA, Olieric N, Aher A, et al.
    Mechanisms of motor-independent membrane remodeling driven by dynamic microtubules
    Current Biology. 2020; 30(6): 972-987. https://doi.org/10.1016/j.cub.2020.01.036
  • Steib E, Laporte MH, Gambarotto D, Olieric N, Zheng C, Borgers S, et al.
    WDR90 is a centriolar microtubule wall protein important for centriole architecture integrity
    eLife. 2020; 9: e57205 (28 pp.). https://doi.org/10.7554/ELIFE.57205
  • Basu S, Olieric V, Leonarski F, Matsugaki N, Kawano Y, Takashi T, et al.
    Long-wavelength native-SAD phasing: opportunities and challenges
    IUCrJ. 2019; 6(3): 1-14. https://doi.org/10.1107/S2052252519002756
  • Brindisi M, Ulivieri C, Alfano G, Gemma S, de Asís Balaguer F, Khan T, et al.
    Structure-activity relationships, biological evaluation and structural studies of novel pyrrolonaphthoxazepines as antitumor agents
    European Journal of Medicinal Chemistry. 2019; 162: 290-320. https://doi.org/10.1016/j.ejmech.2018.11.004
  • Kashyap AS, Fernandez-Rodriguez L, Zhao Y, Monaco G, Trefny MP, Yoshida N, et al.
    GEF-H1 signaling upon microtubule destabilization is required for dendritic cell activation and specific anti-tumor responses
    Cell Reports. 2019; 28(13): 3367-3380.e1. https://doi.org/10.1016/j.celrep.2019.08.057
  • La Sala G, Olieric N, Sharma A, Viti F, de Asis Balaguer Perez F, Huang L, et al.
    Structure, thermodynamics, and kinetics of plinabulin binding to two tubulin isotypes
    Chem. 2019; 5(11): 2969-2986. https://doi.org/10.1016/j.chempr.2019.08.022
  • Wang N, Bosc C, Ryul Choi S, Boulan B, Peris L, Olieric N, et al.
    Structural basis of tubulin detyrosination by the vasohibin–SVBP enzyme complex
    Nature Structural and Molecular Biology. 2019; 26(7): 571-582. https://doi.org/10.1038/s41594-019-0241-y
  • Aher A, Kok M, Sharma A, Rai A, Olieric N, Rodriguez-Garcia R, et al.
    CLASP suppresses microtubule catastrophes through a single TOG domain
    Developmental Cell. 2018; 46(1): 40-58.e8. https://doi.org/10.1016/j.devcel.2018.05.032
  • Lampert F, Stafa D, Goga A, Soste MV, Gilberto S, Olieric N, et al.
    The multi-subunit GID/CTLH e3 ubiquitin ligase promotes cell proliferation and targets the transcription factor Hbp1 for degradation
    eLife. 2018; 7: e35528 (23 pp.). https://doi.org/10.7554/eLife.35528
  • Sharma A, Gerard SF, Olieric N, Steinmetz MO
    Cep120 promotes microtubule formation through a unique tubulin binding C2 domain
    Journal of Structural Biology. 2018; 203(1): 62-70. https://doi.org/10.1016/j.jsb.2018.01.009
  • Smedley CJ, Stanley PA, Qazzaz ME, Prota AE, Olieric N, Collins H, et al.
    Sustainable syntheses of (-)-jerantinines A & E and structural characterisation of the jerantinine-tubulin complex at the colchicine binding site
    Scientific Reports. 2018; 8(1): 10617 (7 pp.). https://doi.org/10.1038/s41598-018-28880-2
  • Aeschimann W, Staats S, Kammer S, Olieric N, Jeckelmann J-M, Fotiadis D, et al.
    Self-assembled α-tocopherol transfer protein nanoparticles promote vitamin E delivery across an endothelial barrier
    Scientific Reports. 2017; 7: 4970 (13 pp.). https://doi.org/10.1038/s41598-017-05148-9
  • Hamel V, Steib E, Hamelin R, Armand F, Borgers S, Flückiger I, et al.
    Identification of Chlamydomonas central core centriolar proteins reveals a role for human WDR90 in ciliogenesis
    Current Biology. 2017; 27(16): 2486-2498. https://doi.org/10.1016/j.cub.2017.07.011
  • Sharma A, Sáez-Calvo G, Olieric N, de Asís Balaguer F, Barasoain I, Lamberth C, et al.
    Quinolin-6-yloxyacetamides are microtubule destabilizing agents that bind to the colchicine site of tubulin
    International Journal of Molecular Sciences. 2017; 18(7): 1336 (11 pp.). https://doi.org/10.3390/ijms18071336
  • Sáez-Calvo G, Sharma A, de Asís Balaguer F, Barasoain I, Rodríguez-Salarichs J, Olieric N, et al.
    Triazolopyrimidines are microtubule-stabilizing agents that bind the vinca inhibitor site of tubulin
    Cell Chemical Biology. 2017; 24(6): 737-750. https://doi.org/10.1016/j.chembiol.2017.05.016
  • Weinert T, Olieric N, Cheng R, Brünle S, James D, Ozerov D, et al.
    Serial millisecond crystallography for routine room-temperature structure determination at synchrotrons
    Nature Communications. 2017; 8(1): 542 (11 pp.). https://doi.org/10.1038/s41467-017-00630-4
  • Bianchi S, van Riel WE, Kraatz SHW, Olieric N, Frey D, Katrukha EA, et al.
    Structural basis for misregulation of kinesin KIF21A autoinhibition by CFEOM1 disease mutations
    Scientific Reports. 2016; 6: 30668 (16 pp.). https://doi.org/10.1038/srep30668
  • Kevenaar JT, Bianchi S, van Spronsen M, Olieric N, Lipka J, Frias CP, et al.
    Kinesin-binding protein controls microtubule dynamics and cargo trafficking by regulating kinesin motor activity
    Current Biology. 2016; 26(7): 849-861. https://doi.org/10.1016/j.cub.2016.01.048
  • Kraatz S, Guichard P, Obbineni JM, Olieric N, Hatzopoulos GN, Hilbert M, et al.
    The human centriolar protein CEP135 contains a two-stranded coiled-coil domain critical for microtubule binding
    Structure. 2016; 24(8): 1358-1371. https://doi.org/10.1016/j.str.2016.06.011
  • Olieric V, Weinert T, Finke AD, Anders C, Li D, Olieric N, et al.
    Data-collection strategy for challenging native SAD phasing
    Acta Crystallographica Section D: Structural Biology. 2016; 72(3): 421-429. https://doi.org/10.1107/S2059798315024110
  • Wieczorek M, Tcherkezian J, Bernier C, Prota AE, Chaaban S, Rolland Y, et al.
    The synthetic diazonamide DZ-2384 has distinct effects on microtubule curvature and dynamics without neurotoxicity
    Science Translational Medicine. 2016; 8(365): 365ra159 (14 pp.). https://doi.org/10.1126/scitranslmed.aag1093
  • Guichard P, Hachet V, Majubu N, Neves A, Demurtas D, Olieric N, et al.
    Native architecture of the centriole proximal region reveals features underlying its 9-fold radial symmetry
    Current Biology. 2013; 23(17): 1620-1628. https://doi.org/10.1016/j.cub.2013.06.061
  • Kitagawa D, Vakonakis I, Olieric N, Hilbert M, Keller D, Olieric V, et al.
    Structural basis of the 9-fold symmetry of centrioles
    Cell. 2011; 144(3): 364 (12 pp.)-375. https://doi.org/10.1016/j.cell.2011.01.008
  • Olieric N, Kuchen M, Wagen S, Sauter M, Crone S, Edmondson S, et al.
    Automated seamless DNA co-transformation cloning with direct expression vectors applying positive or negative insert selection
    BMC Biotechnology. 2010; 10: 56. https://doi.org/10.1186/1472-6750-10-56
  • Bieniossek C, Nie Y, Frey D, Olieric N, Schaffitzel C, Collinson I, et al.
    Automated unrestricted multigene recombineering for multiprotein complex production
    Nature Methods. 2009; 6(6): 447-450. https://doi.org/10.1038/nmeth.1326