Dr. Ashwani Sharma

Scientist
Paul Scherrer Institute
Forschungsstrasse 111
5232 Villigen PSI
Switzerland
Forschungsstrasse 111
5232 Villigen PSI
Switzerland
Telefon
E-Mail
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Kashyap AS, Fernandez-Rodriguez L, Zhao Y, Monaco G, Trefny MP, Yoshida N, et al.
GEF-H1 signaling upon microtubule destabilization is required for dendritic cell activation and specific anti-tumor responses
Cell Reports. 2019; 28(13): 3367-3380.e1. https://doi.org/10.1016/j.celrep.2019.08.057
DORA PSI -
La Sala G, Olieric N, Sharma A, Viti F, de Asis Balaguer Perez F, Huang L, et al.
Structure, thermodynamics, and kinetics of plinabulin binding to two tubulin isotypes
Chem. 2019; 5(11): 2969-2986. https://doi.org/10.1016/j.chempr.2019.08.022
DORA PSI -
Aher A, Kok M, Sharma A, Rai A, Olieric N, Rodriguez-Garcia R, et al.
CLASP suppresses microtubule catastrophes through a single TOG domain
Developmental Cell. 2018; 46(1): 40-58.e8. https://doi.org/10.1016/j.devcel.2018.05.032
DORA PSI -
Sharma A, Gerard SF, Olieric N, Steinmetz MO
Cep120 promotes microtubule formation through a unique tubulin binding C2 domain
Journal of Structural Biology. 2018; 203(1): 62-70. https://doi.org/10.1016/j.jsb.2018.01.009
DORA PSI -
Sharma A, Sáez-Calvo G, Olieric N, de Asís Balaguer F, Barasoain I, Lamberth C, et al.
Quinolin-6-yloxyacetamides are microtubule destabilizing agents that bind to the colchicine site of tubulin
International Journal of Molecular Sciences. 2017; 18(7): 1336 (11 pp.). https://doi.org/10.3390/ijms18071336
DORA PSI -
Sáez-Calvo G, Sharma A, de Asís Balaguer F, Barasoain I, Rodríguez-Salarichs J, Olieric N, et al.
Triazolopyrimidines are microtubule-stabilizing agents that bind the vinca inhibitor site of tubulin
Cell Chemical Biology. 2017; 24(6): 737-750. https://doi.org/10.1016/j.chembiol.2017.05.016
DORA PSI -
Burger D, Stihle M, Sharma A, Di Lello P, Benz J, D'Arcy B, et al.
Crystal structures of the human doublecortin C- and N-terminal domains in complex with specific antibodies
Journal of Biological Chemistry. 2016; 291(31): 16292-16306. https://doi.org/10.1074/jbc.M116.726547
DORA PSI -
Sharma A, Aher A, Dynes NJ, Frey D, Katrukha EA, Jaussi R, et al.
Centriolar CPAP/SAS-4 imparts slow processive microtubule growth
Developmental Cell. 2016; 37(4): 362-376. https://doi.org/10.1016/j.devcel.2016.04.024
DORA PSI
Research Interests
Most of the biological processes are governed by macromolecular complexes of defined composition and organization, which perform specific functions acting as molecular ensembles. Understanding a biological process is essentially an interpretation of the cross-talk between its various components. My main scientific interest lies in deciphering the structural and molecular mechanisms underlying protein-protein interactions involved in complex cellular processes. Microtubules are the major eukaryotic cytoskeletal elements which organize mitotic spindle during cell division and form the core of cellular organelles like centrioles and cilia/flagella. I am currently using biophysical, biochemical and structural methods to understand how microtubule associated proteins and drugs regulate microtubule structure and properties.
Grants/Awards
- Novartis Freenovation Grant
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EMBO Long Term Fellowship
(2012)
Publications
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Quinolin-6-Yloxyacetamides are microtubule destabilizing agents that bind to the colchicine site of Tubulin.
International Journal Of Molecular Sciences 18(7), 1336 (2017).DOI: http://dx.doi.org/10.3390/ijms18071336
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Triazolopyrimidines Are Microtubule-Stabilizing Agents that Bind the Vinca Inhibitor Site of Tubulin.
Cell Chemical Biology 24, 737 (2017).DOI: http://dx.doi.org/10.1016/J.CHEMBIOL.2017.05.016
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Centriolar CPAP/SAS-4 Imparts Slow Processive Microtubule Growth.
Developmental Cell 37(4), 362-376 (2016).DOI: http://dx.doi.org/10.1016/j.devcel.2016.04.024.
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Crystal Structures of the Human Doublecortin C- and N-terminal Domains in Complex with Specific Antibodies.
J Biol Chem. 291(31, 16292-306 (2016).DOI: http://dx.doi.org/10.1074/jbc.M116.726547.
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Purification, crystallization and preliminary crystallographic studies of C-terminal RNA recognition motif (RRM-3) of human ELAV-type RNA-binding protein 3 (ETR-3).
Acta Crystallogr Sect F Struct Biol Cryst Commun. 69(Pt 10), 1107-09 (2013).DOI: http://dx.doi.org/10.1107/S1744309113023439.
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Utility of anion and cation combinations for phasing of protein structures.
J Struct Funct Genomics. 13(3), 135-43 (2012).DOI: http://dx.doi.org/10.1007/s10969-012-9137-3.
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Fatty acid induced remodeling within the human liver fatty acid-binding protein.
J Biol Chem. 286(36), 31924-8 (2011).DOI: http://dx.doi.org/10.1074/jbc.M111.270165.
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Structural insights into thioredoxin-2:a component of malaria parasite protein secretion machinery.
Scientific Reports 1:179, (2011).DOI: http://dx.doi.org/10.1038/srep00179.
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Crystal structure of soluble domain of malaria sporozoite protein UIS3 in complex with lipid.
J Biol Chem. 283(35), 24077-88 (2008).DOI: http://dx.doi.org/10.1074/jbc.M801946200.
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Role of Plasmodium falciparum thrombospondin-related anonymous protein in host-cell interactions.
Malar J. 7:63, (2008).DOI: http://dx.doi.org/10.1186/1475-2875-7-63.