Dr. Ashwani Sharma

Scientist

Paul Scherrer Institute
Forschungsstrasse 111
5232 Villigen PSI
Switzerland
Telephone
+41 56 310 40 47
  • Sharma A, Olieric N, Steinmetz MO
    Centriole length control
    Current Opinion in Structural Biology. 2021; 66: 89-95. https://doi.org/10.1016/j.sbi.2020.10.011
    DORA PSI
  • Kashyap AS, Fernandez-Rodriguez L, Zhao Y, Monaco G, Trefny MP, Yoshida N, et al.
    GEF-H1 signaling upon microtubule destabilization is required for dendritic cell activation and specific anti-tumor responses
    Cell Reports. 2019; 28(13): 3367-3380.e1. https://doi.org/10.1016/j.celrep.2019.08.057
    DORA PSI
  • La Sala G, Olieric N, Sharma A, Viti F, de Asis Balaguer Perez F, Huang L, et al.
    Structure, thermodynamics, and kinetics of plinabulin binding to two tubulin isotypes
    Chem. 2019; 5(11): 2969-2986. https://doi.org/10.1016/j.chempr.2019.08.022
    DORA PSI
  • Aher A, Kok M, Sharma A, Rai A, Olieric N, Rodriguez-Garcia R, et al.
    CLASP suppresses microtubule catastrophes through a single TOG domain
    Developmental Cell. 2018; 46(1): 40-58.e8. https://doi.org/10.1016/j.devcel.2018.05.032
    DORA PSI
  • Sharma A, Gerard SF, Olieric N, Steinmetz MO
    Cep120 promotes microtubule formation through a unique tubulin binding C2 domain
    Journal of Structural Biology. 2018; 203(1): 62-70. https://doi.org/10.1016/j.jsb.2018.01.009
    DORA PSI
  • Sharma A, Sáez-Calvo G, Olieric N, de Asís Balaguer F, Barasoain I, Lamberth C, et al.
    Quinolin-6-yloxyacetamides are microtubule destabilizing agents that bind to the colchicine site of tubulin
    International Journal of Molecular Sciences. 2017; 18(7): 1336 (11 pp.). https://doi.org/10.3390/ijms18071336
    DORA PSI
  • Sáez-Calvo G, Sharma A, de Asís Balaguer F, Barasoain I, Rodríguez-Salarichs J, Olieric N, et al.
    Triazolopyrimidines are microtubule-stabilizing agents that bind the vinca inhibitor site of tubulin
    Cell Chemical Biology. 2017; 24(6): 737-750. https://doi.org/10.1016/j.chembiol.2017.05.016
    DORA PSI
  • Burger D, Stihle M, Sharma A, Di Lello P, Benz J, D'Arcy B, et al.
    Crystal structures of the human doublecortin C- and N-terminal domains in complex with specific antibodies
    Journal of Biological Chemistry. 2016; 291(31): 16292-16306. https://doi.org/10.1074/jbc.M116.726547
    DORA PSI
  • Sharma A, Aher A, Dynes NJ, Frey D, Katrukha EA, Jaussi R, et al.
    Centriolar CPAP/SAS-4 imparts slow processive microtubule growth
    Developmental Cell. 2016; 37(4): 362-376. https://doi.org/10.1016/j.devcel.2016.04.024
    DORA PSI




Most of the biological processes are governed by macromolecular complexes of defined composition and organization, which perform specific functions acting as molecular ensembles. Understanding a biological process is essentially an interpretation of the cross-talk between its various components. My main scientific interest lies in deciphering the structural and molecular mechanisms underlying protein-protein interactions involved in complex cellular processes. Microtubules are the major eukaryotic cytoskeletal elements which organize mitotic spindle during cell division and form the core of cellular organelles like centrioles and cilia/flagella. I am currently using biophysical, biochemical and structural methods to understand how microtubule associated proteins and drugs regulate microtubule structure and properties.

2017

2012

  • EMBO Long Term Fellowship
    (2012)

2017

  • Quinolin-6-Yloxyacetamides are microtubule destabilizing agents that bind to the colchicine site of Tubulin.
    Sharma A, Sáez-Calvo G, Olieric N, de Asís Balaguer F, Barasoain I, Lamberth C, Díaz J, Steinmetz M
    International Journal Of Molecular Sciences 18(7), 1336 (2017).
    DOI: http://dx.doi.org/10.3390/ijms18071336
  • Triazolopyrimidines Are Microtubule-Stabilizing Agents that Bind the Vinca Inhibitor Site of Tubulin.
    Saez-Calvo G, Sharma A, Balaguer FA, Barasoain I, Rodriguez-Salarichs J, Olieric N, Munoz-Hernandez H, Berbis MA, Wendeborn S, Penalva MA, Matesanz R, Canales A, Prota AE, Jimenez-Barbero J, Andreu JM, Lamberth C, Steinmetz MO, Diaz JF
    Cell Chemical Biology 24, 737 (2017).
    DOI: http://dx.doi.org/10.1016/J.CHEMBIOL.2017.05.016

2016

  • Centriolar CPAP/SAS-4 Imparts Slow Processive Microtubule Growth.
    Sharma A, Aher A, Dynes NJ, Frey D, Katrukha EA, Jaussi R, Grigoriev I, Croisier M, Kammerer RA, Akhmanova A, Gönczy P, Steinmetz MO.
    Developmental Cell 37(4), 362-376 (2016).
    DOI: http://dx.doi.org/10.1016/j.devcel.2016.04.024.
  • Crystal Structures of the Human Doublecortin C- and N-terminal Domains in Complex with Specific Antibodies.
    Burger D, Stihle M, Sharma A, Di Lello P, Benz J, D'Arcy B, Debulpaep M, Fry D, Huber W, Kremer T, Laeremans T, Matile H, Ross A, Rufer AC, Schoch G,Steinmetz MO, Steyaert J, Rudolph MG, Thoma R, Ruf A.
    J Biol Chem. 291(31, 16292-306 (2016).
    DOI: http://dx.doi.org/10.1074/jbc.M116.726547.

2013

  • Purification, crystallization and preliminary crystallographic studies of C-terminal RNA recognition motif (RRM-3) of human ELAV-type RNA-binding protein 3 (ETR-3).
    Kashyap M, Sharma A, Bhavesh NS.
    Acta Crystallogr Sect F Struct Biol Cryst Commun. 69(Pt 10), 1107-09 (2013).
    DOI: http://dx.doi.org/10.1107/S1744309113023439.

2012

2011

  • Structural insights into thioredoxin-2:a component of malaria parasite protein secretion machinery.
    Sharma A, Sharma A, Dixit S, Sharma A.
    Scientific Reports 1:179, (2011).
    DOI: http://dx.doi.org/10.1038/srep00179.

2008

  • Crystal structure of soluble domain of malaria sporozoite protein UIS3 in complex with lipid.
    Sharma A, Yogavel M, Akhouri RR, Gill J, Sharma A.
    J Biol Chem. 283(35), 24077-88 (2008).
    DOI: http://dx.doi.org/10.1074/jbc.M801946200.
  • Role of Plasmodium falciparum thrombospondin-related anonymous protein in host-cell interactions.
    Akhouri RR, Sharma A, Malhotra P, Sharma A.
    Malar J. 7:63, (2008).
    DOI: http://dx.doi.org/10.1186/1475-2875-7-63.